000819577 001__ 819577
000819577 005__ 20240619091212.0
000819577 037__ $$aFZJ-2016-05207
000819577 1001_ $$0P:(DE-Juel1)164319$$aKreysing, Eva$$b0$$eCorresponding author$$ufzj
000819577 245__ $$aPoster Award "Surface Plasmon Resonance Microscopy of the Cell-Chip Interface"
000819577 260__ $$aGermany$$bGerman Biophysics Society
000819577 3367_ $$2DataCite$$aOther
000819577 3367_ $$2EndNote$$aGrant
000819577 3367_ $$2BibTeX$$aMISC
000819577 3367_ $$0PUB:(DE-HGF)38$$2PUB:(DE-HGF)$$aAward$$baward$$maward$$s1482229230_9862
000819577 3367_ $$2ORCID$$aOTHER
000819577 3367_ $$2DINI$$aOther
000819577 502__ $$d2016
000819577 520__ $$aPoster: "Surface Plasmon Resonance Microscopy of the Cell-Chip Interface"Longterm investigation of neuronal networks require non-invasive recordings of the electrical signals. A good coupling between the biological and electronic system is crucial and depends particularly upon the  cell-chip  distance.  The  cell-chip  distance  is  an  important  parameter  towards  a  good  sealing,  with closer contact leading to a decreased signal dissipation in the cell-electrode cleft. We therefore try to optimize  the  contact  geometry  of  said  interface  using  protein  and  lipid  coatings.  In  order  to  measure the  distances  between  the  cell  membrane  and  the  chip  surface  in  vitro,  we  built  a  surface  plasmon resonance microscope (SPRM). With gold coated sapphire chips as the substrate for the cell culture, it is possible to excite plasmons (collective  electron  oscillations)  in  the  gold  layer  by  illuminating  it  under  a  specific  angle.  The resonance  frequency  of  the  plasmons  depends  strongly  upon  the  dielectric  constant  of  the  gold's environment.  In  turn  the  angle  spectrum  of  the  reflected  light  depends upon  said  resonance frequencies. Due to these dependencies it is possible to deduce the cell-substrate distance.Our microscope is capable of imaging the interface in two different modes. The field of view in the live imaging  mode  is  around  65  um  x  65  um.This  is  useful  for  determining  the  region  of  interest  for  the scanning  mode.  This  mode  uses  localized  surface  plasmons  to  measure  the  cell-substrate  distance. The resolution in z-direction lies in the nanometer range. This allows us to accurately characterize the cell-chip interface.Since SPRM is non-invasive and label free it is suited for longterm investigations.It is therefore possible to observe the development of neuronal networks over several week.
000819577 536__ $$0G:(DE-HGF)POF3-552$$a552 - Engineering Cell Function (POF3-552)$$cPOF3-552$$fPOF III$$x0
000819577 909CO $$ooai:juser.fz-juelich.de:819577$$pVDB
000819577 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)164319$$aForschungszentrum Jülich$$b0$$kFZJ
000819577 9131_ $$0G:(DE-HGF)POF3-552$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vEngineering Cell Function$$x0
000819577 920__ $$lyes
000819577 9201_ $$0I:(DE-Juel1)ICS-8-20110106$$kICS-8$$lBioelektronik$$x0
000819577 980__ $$aaward
000819577 980__ $$aVDB
000819577 980__ $$aI:(DE-Juel1)ICS-8-20110106
000819577 980__ $$aUNRESTRICTED
000819577 981__ $$aI:(DE-Juel1)IBI-3-20200312