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000819671 1001_ $$0P:(DE-HGF)0$$aSafarik, Ivo$$b0$$eCorresponding author
000819671 245__ $$aComposite particles formed by complexation of poly(methacrylic acid) — stabilized magnetic fluid with chitosan: Magnetic material for bioapplications
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000819671 520__ $$aA simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
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000819671 7001_ $$0P:(DE-HGF)0$$aStepanek, Miroslav$$b1
000819671 7001_ $$0P:(DE-HGF)0$$aUchman, Mariusz$$b2
000819671 7001_ $$0P:(DE-HGF)0$$aSlouf, Miroslav$$b3
000819671 7001_ $$0P:(DE-HGF)0$$aBaldikova, Eva$$b4
000819671 7001_ $$0P:(DE-HGF)0$$aNydlova, Leona$$b5
000819671 7001_ $$0P:(DE-HGF)0$$aPospiskova, Kristyna$$b6
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000819671 773__ $$0PERI:(DE-600)2012160-X$$a10.1016/j.msec.2016.05.017$$gVol. 67, p. 486 - 492$$p486 - 492$$tMaterials science and engineering / C$$v67$$x0928-4931$$y2016
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