%0 Journal Article
%A Akone, Sergi Herve
%A Mándi, Attila
%A Kurtán, Tibor
%A Hartmann, Rudolf
%A Lin, Wenhan
%A Daletos, Georgios
%A Proksch, Peter
%T Inducing secondary metabolite production by the endophytic fungus Chaetomium sp. through fungal–bacterial co-culture and epigenetic modification
%J Tetrahedron
%V 72
%N 41
%@ 0040-4020
%C Amsterdam [u.a.]
%I Elsevier Science
%M FZJ-2016-05397
%P 6340 - 6347
%D 2016
%X Co-culturing the fungal endophyte Chaetomium sp. with the bacterium Bacillus subtilis on solid rice medium resulted in an up to 8.3-fold increase in the accumulation of constitutively present metabolites that included a 1:1 mixture of 3- and 4-hydroxybenzoic acid methyl esters (1 and 2, respectively), and the polyketides acremonisol A (3), SB236050 (4), and SB238569 (5). In addition, seven compounds including isosulochrin (6), protocatechuic acid methyl ester (7), as well as five new natural products (8e12) were detected in the co-cultures, but not in axenic fungal cultures. Treatment of Chaetomium sp. with the epigenetic modifier suberoylanilide hydroxamic acid or 5-azacytidine resulted in an enhanced accumulation of 6, which was likewise detected during co-culture. Compound 5 showed strong cytotoxicity against the mouse lymphoma L5178Y cell line with an IC50 value of 1 mM, as well as weak antibacterial activity against B. subtilis with an MIC value of 53 mM.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000384776300007
%R 10.1016/j.tet.2016.08.022
%U https://juser.fz-juelich.de/record/819802