TY  - JOUR
AU  - Akone, Sergi Herve
AU  - Mándi, Attila
AU  - Kurtán, Tibor
AU  - Hartmann, Rudolf
AU  - Lin, Wenhan
AU  - Daletos, Georgios
AU  - Proksch, Peter
TI  - Inducing secondary metabolite production by the endophytic fungus Chaetomium sp. through fungal–bacterial co-culture and epigenetic modification
JO  - Tetrahedron
VL  - 72
IS  - 41
SN  - 0040-4020
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2016-05397
SP  - 6340 - 6347
PY  - 2016
AB  - Co-culturing the fungal endophyte Chaetomium sp. with the bacterium Bacillus subtilis on solid rice medium resulted in an up to 8.3-fold increase in the accumulation of constitutively present metabolites that included a 1:1 mixture of 3- and 4-hydroxybenzoic acid methyl esters (1 and 2, respectively), and the polyketides acremonisol A (3), SB236050 (4), and SB238569 (5). In addition, seven compounds including isosulochrin (6), protocatechuic acid methyl ester (7), as well as five new natural products (8e12) were detected in the co-cultures, but not in axenic fungal cultures. Treatment of Chaetomium sp. with the epigenetic modifier suberoylanilide hydroxamic acid or 5-azacytidine resulted in an enhanced accumulation of 6, which was likewise detected during co-culture. Compound 5 showed strong cytotoxicity against the mouse lymphoma L5178Y cell line with an IC50 value of 1 mM, as well as weak antibacterial activity against B. subtilis with an MIC value of 53 mM.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000384776300007
DO  - DOI:10.1016/j.tet.2016.08.022
UR  - https://juser.fz-juelich.de/record/819802
ER  -