%0 Journal Article
%A Liu, Shuai
%A Dai, Haofu
%A Makhloufi, Gamall
%A Heering, Christian
%A Janiak, Christoph
%A Hartmann, Rudolf
%A Mándi, Attila
%A Kurtán, Tibor
%A Müller, Werner E. G.
%A Kassack, Matthias U.
%A Lin, Wenhan
%A Liu, Zhen
%A Proksch, Peter
%T Cytotoxic 14-Membered Macrolides from a Mangrove-Derived Endophytic Fungus, Pestalotiopsis microspora
%J Journal of natural products
%V 79
%N 9
%@ 1520-6025
%C Washington, DC
%I Soc.
%M FZJ-2016-05401
%P 2332 - 2340
%D 2016
%X Seven new 14-membered macrolides, pestalotioprolides C (2), D−H (4−8), and 7-O-methylnigrosporolide (3), together with four known analogues, pestalotioprolide B (1), seiricuprolide (9), nigrosporolide (10), and 4,7-dihydroxy-13-tetradeca-2,5,8-trienolide (11), were isolated from the mangrove-derived endophytic fungus Pestalotiopsis microspora. Their structures were elucidated by analysis of NMR and MS data and by comparison with literature data. Single-crystal X-ray diffraction analysis was used to confirm the absolute configurations of 1, 2, and 10, while Mosher’s method and the TDDFT-ECD approach were applied to determine the absolute configurations of 5 and 6. Compounds 3−6 showed significant cytotoxicity against the murine lymphoma cell line L5178Y with IC50 values of 0.7, 5.6, 3.4, and 3.9 μM, respectively, while compound 5 showed potent activity against the human ovarian cancer cell line A2780 with an IC50 value of 1.2 μM. Structure−activity relationships are discussed. Coculture of P. microspora with Streptomyces lividans caused a roughly 10-fold enhanced accumulation of compounds 5 and 6 compared to axenic fungal control.
%F PUB:(DE-HGF)16
%9 Journal Article
%U <Go to ISI:>//WOS:000384156700024
%$ pmid:27556865
%R 10.1021/acs.jnatprod.6b00473
%U https://juser.fz-juelich.de/record/819806