TY  - JOUR
AU  - Liu, Shuai
AU  - Dai, Haofu
AU  - Makhloufi, Gamall
AU  - Heering, Christian
AU  - Janiak, Christoph
AU  - Hartmann, Rudolf
AU  - Mándi, Attila
AU  - Kurtán, Tibor
AU  - Müller, Werner E. G.
AU  - Kassack, Matthias U.
AU  - Lin, Wenhan
AU  - Liu, Zhen
AU  - Proksch, Peter
TI  - Cytotoxic 14-Membered Macrolides from a Mangrove-Derived Endophytic Fungus, Pestalotiopsis microspora
JO  - Journal of natural products
VL  - 79
IS  - 9
SN  - 1520-6025
CY  - Washington, DC
PB  - Soc.
M1  - FZJ-2016-05401
SP  - 2332 - 2340
PY  - 2016
AB  - Seven new 14-membered macrolides, pestalotioprolides C (2), D−H (4−8), and 7-O-methylnigrosporolide (3), together with four known analogues, pestalotioprolide B (1), seiricuprolide (9), nigrosporolide (10), and 4,7-dihydroxy-13-tetradeca-2,5,8-trienolide (11), were isolated from the mangrove-derived endophytic fungus Pestalotiopsis microspora. Their structures were elucidated by analysis of NMR and MS data and by comparison with literature data. Single-crystal X-ray diffraction analysis was used to confirm the absolute configurations of 1, 2, and 10, while Mosher’s method and the TDDFT-ECD approach were applied to determine the absolute configurations of 5 and 6. Compounds 3−6 showed significant cytotoxicity against the murine lymphoma cell line L5178Y with IC50 values of 0.7, 5.6, 3.4, and 3.9 μM, respectively, while compound 5 showed potent activity against the human ovarian cancer cell line A2780 with an IC50 value of 1.2 μM. Structure−activity relationships are discussed. Coculture of P. microspora with Streptomyces lividans caused a roughly 10-fold enhanced accumulation of compounds 5 and 6 compared to axenic fungal control.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000384156700024
C6  - pmid:27556865
DO  - DOI:10.1021/acs.jnatprod.6b00473
UR  - https://juser.fz-juelich.de/record/819806
ER  -