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@ARTICLE{Liu:819806,
author = {Liu, Shuai and Dai, Haofu and Makhloufi, Gamall and
Heering, Christian and Janiak, Christoph and Hartmann,
Rudolf and Mándi, Attila and Kurtán, Tibor and Müller,
Werner E. G. and Kassack, Matthias U. and Lin, Wenhan and
Liu, Zhen and Proksch, Peter},
title = {{C}ytotoxic 14-{M}embered {M}acrolides from a
{M}angrove-{D}erived {E}ndophytic {F}ungus, {P}estalotiopsis
microspora},
journal = {Journal of natural products},
volume = {79},
number = {9},
issn = {1520-6025},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2016-05401},
pages = {2332 - 2340},
year = {2016},
abstract = {Seven new 14-membered macrolides, pestalotioprolides C (2),
D−H (4−8), and 7-O-methylnigrosporolide (3), together
with four known analogues, pestalotioprolide B (1),
seiricuprolide (9), nigrosporolide (10), and
4,7-dihydroxy-13-tetradeca-2,5,8-trienolide (11), were
isolated from the mangrove-derived endophytic fungus
Pestalotiopsis microspora. Their structures were elucidated
by analysis of NMR and MS data and by comparison with
literature data. Single-crystal X-ray diffraction analysis
was used to confirm the absolute configurations of 1, 2, and
10, while Mosher’s method and the TDDFT-ECD approach were
applied to determine the absolute configurations of 5 and 6.
Compounds 3−6 showed significant cytotoxicity against the
murine lymphoma cell line L5178Y with IC50 values of 0.7,
5.6, 3.4, and 3.9 μM, respectively, while compound 5 showed
potent activity against the human ovarian cancer cell line
A2780 with an IC50 value of 1.2 μM. Structure−activity
relationships are discussed. Coculture of P. microspora with
Streptomyces lividans caused a roughly 10-fold enhanced
accumulation of compounds 5 and 6 compared to axenic fungal
control.},
cin = {ICS-6},
ddc = {500},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000384156700024},
pubmed = {pmid:27556865},
doi = {10.1021/acs.jnatprod.6b00473},
url = {https://juser.fz-juelich.de/record/819806},
}