TY  - JOUR
AU  - Viegas, Aldino
AU  - Viennet, Thibault
AU  - Yu, Tsyr-Yan
AU  - Schumann, Frank
AU  - Bermel, Wolfgang
AU  - Wagner, Gerhard
AU  - Etzkorn, Manuel
TI  - UTOPIA NMR: activating unexploited magnetization using interleaved low-gamma detection
JO  - Journal of biomolecular NMR
VL  - 64
IS  - 1
SN  - 1573-5001
CY  - Dordrecht [u.a.]
PB  - Springer Science + Business Media B.V
M1  - FZJ-2016-05502
SP  - 9 - 15
PY  - 2016
AB  - A growing number of nuclear magnetic resonance (NMR) spectroscopic studies are impaired by the limited information content provided by the standard set of experiments conventionally recorded. This is particularly true for studies of challenging biological systems including large, unstructured, membrane-embedded and/or paramagnetic proteins. Here we introduce the concept of unified time-optimized interleaved acquisition NMR (UTOPIANMR) for the unified acquisition of standard high-c (e.g. 1H) and low-c (e.g. 13C) detected experiments using a single receiver. Our aim is to activate the high level of polarization and information content distributed on low-c nuclei without disturbing conventional magnetization transfer pathways. We show that using UTOPIA-NMR we are able to recover nearly all of the normally non-used magnetization without disturbing the standard experiments. In other words, additional spectra, that can significantly increase the NMR insights, are obtained for free. While we anticipate a broad range of possible applications we demonstrate for the soluble protein Bcl-xL (ca. 21 kDa) and for OmpX in nanodiscs (ca. 160 kDa) that UTOPIA-NMR is particularly useful for challenging protein systems including perdeuterated (membrane) proteins.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000372168000002
DO  - DOI:10.1007/s10858-015-0008-7
UR  - https://juser.fz-juelich.de/record/819928
ER  -