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@ARTICLE{Manning:819957,
      author       = {Manning, Grainne and Macaeva, Ellina and Majewski,
                      Matthaeus and Kriehuber, Ralf and Brzóska, Kamil and Abend,
                      Michael and Doucha-Senf, Sven and Oskamp, Dominik and
                      Strunz, Sonja and Quintens, Roel and Port, Matthias and
                      Badie, Christophe},
      title        = {{C}omparable dose estimates of blinded whole blood samples
                      are obtained independently of culture conditions and
                      analytical approaches. {S}econd {RENEB} gene expression
                      study},
      journal      = {International journal of radiation biology},
      volume       = {93},
      number       = {1},
      issn         = {1362-3095},
      address      = {Abingdon},
      publisher    = {Informa Healthcare84105},
      reportid     = {FZJ-2016-05524},
      pages        = {87-98},
      year         = {2017},
      abstract     = {PURPOSE:This collaboration of five established European
                      gene expression labs investigated the potential impact of
                      culture conditions on the transcriptional response of
                      peripheral blood to radiation exposure.MATERIALS AND
                      METHODS:Blood from one healthy donor was exposed ex vivo to
                      a Cobalt 60 source to produce a calibration curve in
                      addition to four unknown doses. After exposure, the blood
                      samples were either diluted with RPMI medium or left
                      untouched. After 24-h incubation at 37 °C the diluted
                      blood samples were lysed, while the undiluted samples were
                      mixed with the preservative RNALater and all samples were
                      shipped frozen to the participating labs. Samples were
                      processed by each lab using microarray (one lab) and QRT-PCR
                      (four labs).RESULTS:We show that although culture conditions
                      affect the total amount of RNA recovered (p < .0001) and
                      its integrity (p < .0001), it does not significantly
                      affect dose estimates (except for the true dose at
                      1.1 Gy). Most importantly, the different analysis
                      approaches provide comparable mean absolute difference of
                      estimated doses relative to the true doses (p = .9) and
                      number of out of range (>0.5 Gy) measurements
                      (p = .6).CONCLUSION:This study confirms the robustness
                      of gene expression as a method for biological dosimetry.},
      cin          = {S-US},
      ddc          = {570},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000392891400012},
      pubmed       = {pmid:27626709},
      doi          = {10.1080/09553002.2016.1227105},
      url          = {https://juser.fz-juelich.de/record/819957},
}