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@ARTICLE{Chen:820381,
author = {Chen, La and Maybeck, Vanessa and Offenhäusser, Andreas
and Krause, Hans-Joachim},
title = {{I}mplementation and application of a novel 2{D} magnetic
twisting cytometry based on multi-pole elctromagnet},
journal = {Review of scientific instruments},
volume = {87},
number = {6},
issn = {1089-7623},
address = {[S.l.]},
publisher = {American Institute of Physics},
reportid = {FZJ-2016-05712},
pages = {064301 -},
year = {2016},
abstract = {We implemented a novel 2D magnetic twisting cytometry (MTC)
based on a previously reported multi-pole high permeability
electromagnet, in which both the strength and direction of
the twisting field can be controlled. Thanks to the high
performance twisting electromagnet and the heterodyning
technology, the measurement frequency has been extended to
the 1 kHz range. In order to obtain high remanence of the
ferromagnetic beads, a separate electromagnet with feedback
control was adopted for the high magnetic field
polarization. Our setup constitutes the first instrument
which can be operated both in MTC mode and in magnetic
tweezers (MT) mode. In this work, the mechanical properties
of HL-1 cardiomyocytes were characterized in MTC mode. Both
anisotropy and log-normal distribution of cell stiffness
were observed, which agree with our previous results
measured in MT mode. The response from these living cells at
different frequencies can be fitted very well by the soft
glassy rheology model.},
cin = {ICS-8},
ddc = {530},
cid = {I:(DE-Juel1)ICS-8-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000379177000059},
doi = {10.1063/1.4954185},
url = {https://juser.fz-juelich.de/record/820381},
}