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@ARTICLE{Henry:820533,
author = {Henry, Ewan and Holm, Stefan H. and Zhang, Zunmin and
Beech, Jason P. and Tegenfeldt, Jonas O. and Fedosov, Dmitry
and Gompper, Gerhard},
title = {{S}orting cells by their dynamical properties},
journal = {Scientific reports},
volume = {6},
issn = {2045-2322},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2016-05822},
pages = {34375},
year = {2016},
abstract = {Recent advances in cell sorting aim at the development of
novel methods that are sensitive to various mechanical
properties of cells. Microfluidic technologies have a great
potential for cell sorting; however, the design of many
micro-devices is based on theories developed for rigid
spherical particles with size as a separation parameter.
Clearly, most bioparticles are non-spherical and deformable
and therefore exhibit a much more intricate behavior in
fluid flow than rigid spheres. Here, we demonstrate the use
of cells’ mechanical and dynamical properties as
biomarkers for separation by employing a combination of
mesoscale hydrodynamic simulations and microfluidic
experiments. The dynamic behavior of red blood cells (RBCs)
within deterministic lateral displacement (DLD) devices is
investigated for different device geometries and viscosity
contrasts between the intra-cellular fluid and suspending
medium. We find that the viscosity contrast and associated
cell dynamics clearly determine the RBC trajectory through a
DLD device. Simulation results compare well to experiments
and provide new insights into the physical mechanisms which
govern the sorting of non-spherical and deformable cells in
DLD devices. Finally, we discuss the implications of cell
dynamics for sorting schemes based on properties other than
cell size, such as mechanics and morphology.},
cin = {ICS-2 / JARA-HPC},
ddc = {000},
cid = {I:(DE-Juel1)ICS-2-20110106 / $I:(DE-82)080012_20140620$},
pnm = {552 - Engineering Cell Function (POF3-552) / Blood Flow
Resistance in Microvascular Networks $(jics21_20131101)$},
pid = {G:(DE-HGF)POF3-552 / $G:(DE-Juel1)jics21_20131101$},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000385171400001},
pubmed = {pmid:27708337},
doi = {10.1038/srep34375},
url = {https://juser.fz-juelich.de/record/820533},
}