% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Gaspar:8207,
      author       = {Gaspar, A.M. and Busch, S. and Appavou, M.-S. and
                      Haeussler, W. and Georgii, R. and Su, Y. and Doster, W.},
      title        = {{U}sing polarization analysis to separate the coherent and
                      incoherent scattering from protein samples},
      journal      = {Biochimica et biophysica acta},
      volume       = {1804},
      issn         = {0006-3002},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {PreJuSER-8207},
      pages        = {76 - 82},
      year         = {2010},
      note         = {A. M. Gaspar acknowledges the support given by Fundacao
                      para Ciencia e Tecnologia in the form of a post-doc grant
                      SFRH/BDP/17571/2004. The project was further supported by a
                      grant of the Deutsche Forschungsgemeinschaft SFB 533.},
      abstract     = {Polarization analysis was used to separate experimentally
                      the coherent and spin-incoherent nuclear static scattering
                      functions, from a representative set of samples of interest
                      for protein studies. This method had so far limited
                      application in the study of amorphous materials, despite the
                      relevance of the information that it provides. It allows,
                      for instance, the experimental determination of the
                      structure factor of materials containing a significant
                      amount of hydrogen atoms, avoiding the contamination of
                      measurements by a non-negligible incoherent background.
                      Knowledge of the relative importance of the coherent and
                      incoherent terms at different Q-values is also a
                      pre-requisite for the interpretation of quasielastic neutron
                      scattering experiments, performed at instruments in which
                      the total dynamic scattering function is measured, such as
                      conventional time-of-flight and backscattering
                      spectrometers. Combining data from different instruments, it
                      was possible to cover a wide Q-range, from the small-angle
                      region (0.006<Q<0.04 A(-1)) to the wide-angle region (up to
                      approximately 2.35 A(-1)). Quantitative information was
                      obtained on the fraction of coherent to spin-incoherent
                      scattering from different protein samples: deuterated and
                      protonated protein powders at different hydration levels and
                      solutions of protonated proteins in D(2)O at different
                      concentrations. The results obtained are discussed in the
                      context of the validity of the assumptions generally made
                      when interpreting quasielastic neutron scattering
                      experiments performed without polarization analysis.},
      keywords     = {Deuterium Oxide: chemistry / Hemoglobins: chemistry /
                      Models, Chemical / Myoglobin: chemistry / Neutron
                      Diffraction: methods / Phycocyanin: chemistry / Proteins:
                      chemistry / Solutions / Water: chemistry / Hemoglobins (NLM
                      Chemicals) / Myoglobin (NLM Chemicals) / Proteins (NLM
                      Chemicals) / Solutions (NLM Chemicals) / Phycocyanin (NLM
                      Chemicals) / Water (NLM Chemicals) / Deuterium Oxide (NLM
                      Chemicals) / J (WoSType)},
      cin          = {IFF-4 / IFF-5 / Jülich Centre for Neutron Science JCNS
                      (JCNS) ; JCNS},
      ddc          = {570},
      cid          = {I:(DE-Juel1)VDB784 / I:(DE-Juel1)VDB785 /
                      I:(DE-Juel1)JCNS-20121112},
      pnm          = {BioSoft: Makromolekulare Systeme und biologische
                      Informationsverarbeitung / Großgeräte für die Forschung
                      mit Photonen, Neutronen und Ionen (PNI)},
      pid          = {G:(DE-Juel1)FUEK505 / G:(DE-Juel1)FUEK415},
      experiment   = {EXP:(DE-MLZ)DNS-20140101 / EXP:(DE-MLZ)MIRA-20140101 /
                      EXP:(DE-MLZ)RESEDA-20140101},
      shelfmark    = {Biochemistry $\&$ Molecular Biology / Biophysics},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:19595800},
      UT           = {WOS:000272765200012},
      doi          = {10.1016/j.bbapap.2009.06.024},
      url          = {https://juser.fz-juelich.de/record/8207},
}