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024 7 _ |2 DOI
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|a GasparBAHGSD2009
037 _ _ |a PreJuSER-8207
041 _ _ |a eng
082 _ _ |a 570
084 _ _ |2 WoS
|a Biochemistry & Molecular Biology
084 _ _ |2 WoS
|a Biophysics
100 1 _ |0 P:(DE-HGF)0
|a Gaspar, A.M.
|b 0
245 _ _ |a Using polarization analysis to separate the coherent and incoherent scattering from protein samples
260 _ _ |a Amsterdam [u.a.]
|b Elsevier
|c 2010
300 _ _ |a 76 - 82
336 7 _ |a Journal Article
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336 7 _ |a JOURNAL_ARTICLE
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336 7 _ |a article
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440 _ 0 |0 802
|a Biochimica et Biophysica Acta
|v 1804
|x 0006-3002
|y 1
500 _ _ |a A. M. Gaspar acknowledges the support given by Fundacao para Ciencia e Tecnologia in the form of a post-doc grant SFRH/BDP/17571/2004. The project was further supported by a grant of the Deutsche Forschungsgemeinschaft SFB 533.
520 _ _ |a Polarization analysis was used to separate experimentally the coherent and spin-incoherent nuclear static scattering functions, from a representative set of samples of interest for protein studies. This method had so far limited application in the study of amorphous materials, despite the relevance of the information that it provides. It allows, for instance, the experimental determination of the structure factor of materials containing a significant amount of hydrogen atoms, avoiding the contamination of measurements by a non-negligible incoherent background. Knowledge of the relative importance of the coherent and incoherent terms at different Q-values is also a pre-requisite for the interpretation of quasielastic neutron scattering experiments, performed at instruments in which the total dynamic scattering function is measured, such as conventional time-of-flight and backscattering spectrometers. Combining data from different instruments, it was possible to cover a wide Q-range, from the small-angle region (0.006
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650 _ 2 |2 MeSH
|a Deuterium Oxide: chemistry
650 _ 2 |2 MeSH
|a Hemoglobins: chemistry
650 _ 2 |2 MeSH
|a Models, Chemical
650 _ 2 |2 MeSH
|a Myoglobin: chemistry
650 _ 2 |2 MeSH
|a Neutron Diffraction: methods
650 _ 2 |2 MeSH
|a Phycocyanin: chemistry
650 _ 2 |2 MeSH
|a Proteins: chemistry
650 _ 2 |2 MeSH
|a Solutions
650 _ 2 |2 MeSH
|a Water: chemistry
650 _ 7 |0 0
|2 NLM Chemicals
|a Hemoglobins
650 _ 7 |0 0
|2 NLM Chemicals
|a Myoglobin
650 _ 7 |0 0
|2 NLM Chemicals
|a Proteins
650 _ 7 |0 0
|2 NLM Chemicals
|a Solutions
650 _ 7 |0 11016-15-2
|2 NLM Chemicals
|a Phycocyanin
650 _ 7 |0 7732-18-5
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|a Water
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|a Deuterium Oxide
650 _ 7 |2 WoSType
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653 2 0 |2 Author
|a Neutron scattering
653 2 0 |2 Author
|a Polarization analysis
653 2 0 |2 Author
|a Coherent/incoherent nuclear scattering
653 2 0 |2 Author
|a Protonated/deuterated protein
653 2 0 |2 Author
|a Structure
653 2 0 |2 Author
|a Dynamic
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856 7 _ |u http://dx.doi.org/10.1016/j.bbapap.2009.06.024
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Marc 21