% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kong:820869,
      author       = {Kong, Xianwang and Duan, Yunfeng and Schramm, Andreas and
                      Eriksen, Jørgen and Holmstrup, Martin and Larsen, Thomas
                      and Bol, Roland and Petersen, Søren O.},
      title        = {{M}itigating {N}$_{2}${O} emissions from clover residues by
                      3,4-dimethylpyrazole phosphate ({DMPP}) without adverse
                      effects on the earthworm {L}umbricus terrestris},
      journal      = {Soil biology $\&$ biochemistry},
      volume       = {104},
      issn         = {0038-0717},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2016-06133},
      pages        = {95 - 107},
      year         = {2017},
      abstract     = {In EU-28, temporary grasslands constitute more than $10\%$
                      of the total arable land. Grassland tillage will return up
                      to 400 kg N ha−1 in residues that can lead to a pulse of
                      N2O emissions. Here a novel application of the nitrification
                      inhibitor 3,4-dimethylpyrazole phosphate (DMPP) was
                      evaluated in a 28-d mesocosm experiment, where DMPP spraying
                      prior to tillage was simulated. Above-ground parts of
                      15N-labelled clover residues were treated with DMPP and
                      either placed at 10 cm depth to simulate ploughing (PL), or
                      mixed with soil at 0–10 cm depth to simulate rotovation
                      (RO). Earthworms (Lumbricus terrestris) were introduced to
                      study their role in residue decomposition and N2O emissions.
                      Fluxes and isotopic composition of N2O were determined with
                      dynamic chambers using laser spectroscopy. A gradual
                      increase in 15N-enrichment of N2O indicated that
                      denitrification was the main source. DMPP reduced cumulative
                      N2O emissions in PL from 241 to 146 mg N m−2; the
                      reduction in RO was smaller, from 103 to 94 mg N m−2, and
                      not significant, possibly due to higher oxygen and soil
                      NO3− availability. After 28 d incubation, on average >
                      $90\%$ of the earthworms were recovered, and in vivo N2O
                      production from L. terrestris accounted for only $1–6\%$
                      of residue-induced emissions. The guts were removed from
                      worms to quantify DNA and mRNA of denitrifying genes (nirK,
                      nirS, nosZ clade I and II). It was found that earthworm in
                      vivo N2O production rates were negatively correlated to mRNA
                      of nosZ-I, but positively to nir/nos ratio, suggesting the
                      active involvement of denitrifiers associated with ingested
                      feed in these emissions. Earthworm body tissue was enriched
                      with 15N, with no effect of DMPP. This showed that the worms
                      had fed on the clover residues, and that DMPP did not
                      interfere with feeding behavior or nitrogen assimilation.
                      The observed effects show that DMPP treatment of clover
                      residue before tillage has the potential to modify N2O
                      emissions without adverse effects on residue decomposition
                      or soil fauna.},
      cin          = {IBG-3},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {255 - Terrestrial Systems: From Observation to Prediction
                      (POF3-255)},
      pid          = {G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000389555900010},
      doi          = {10.1016/j.soilbio.2016.10.012},
      url          = {https://juser.fz-juelich.de/record/820869},
}