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@ARTICLE{Scheer:820892,
      author       = {Scheer, Clemens and Meier, Rudolf and Brüggemann, Nicolas
                      and Grace, Peter R. and Dannenmann, Michael},
      title        = {{A}n improved $^{15}$ {N} tracer approach to study
                      denitrification and nitrogen turnover in soil incubations},
      journal      = {Rapid communications in mass spectrometry},
      volume       = {30},
      number       = {18},
      issn         = {0951-4198},
      address      = {New York, NY},
      publisher    = {Wiley Interscience},
      reportid     = {FZJ-2016-06156},
      pages        = {2017 - 2026},
      year         = {2016},
      abstract     = {RationaleDenitrification (the reduction of oxidized forms
                      of inorganic nitrogen (N) to N2O and N2) from upland soils
                      is considered to be the least well-understood process in the
                      global N cycle. The main reason for this lack of
                      understanding is that the terminal product (N2) of
                      denitrification is extremely difficult to measure against
                      the large atmospheric background.MethodsWe describe a system
                      that combines the 15N-tracer technique with a 40-fold
                      reduced N2 $(2\%$ v/v) atmosphere in a fully automated
                      incubation setup for direct quantification of N2 and N2O
                      emissions. The δ15N values of the emitted N2 and N2O were
                      determined using a custom-built gas preparation unit that
                      was connected to a DELTA V Plus isotope ratio mass
                      spectrometer. The system was tested on a pasture soil from
                      sub-tropical Australia under different soil moisture
                      conditions and combined with 15N tracing in extractable soil
                      N pools to establish a full N balance.ResultsThe method
                      proved to be highly sensitive for detecting N2 (1.12 μg N
                      h−1 kg−1 dry soil (ds)) and N2O (0.36 μg N h−1 kg−1
                      ds) emissions. The main end product of denitrification in
                      the investigated soil was N2O for both water contents, with
                      N2 accounting for only $3\%$ to $13\%$ of the total
                      denitrification losses. Between 90 and $95\%$ of the added
                      15N fertiliser could be recovered in N gases and extractable
                      soil N pools.},
      cin          = {IBG-3},
      ddc          = {530},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {255 - Terrestrial Systems: From Observation to Prediction
                      (POF3-255)},
      pid          = {G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000382976800004},
      pubmed       = {pmid:27470312},
      doi          = {10.1002/rcm.7689},
      url          = {https://juser.fz-juelich.de/record/820892},
}