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024 7 _ |a 10.1002/rcm.7689
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024 7 _ |a 1097-0231
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082 _ _ |a 530
100 1 _ |a Scheer, Clemens
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|e Corresponding author
245 _ _ |a An improved $^{15}$ N tracer approach to study denitrification and nitrogen turnover in soil incubations
260 _ _ |a New York, NY
|c 2016
|b Wiley Interscience
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520 _ _ |a RationaleDenitrification (the reduction of oxidized forms of inorganic nitrogen (N) to N2O and N2) from upland soils is considered to be the least well-understood process in the global N cycle. The main reason for this lack of understanding is that the terminal product (N2) of denitrification is extremely difficult to measure against the large atmospheric background.MethodsWe describe a system that combines the 15N-tracer technique with a 40-fold reduced N2 (2% v/v) atmosphere in a fully automated incubation setup for direct quantification of N2 and N2O emissions. The δ15N values of the emitted N2 and N2O were determined using a custom-built gas preparation unit that was connected to a DELTA V Plus isotope ratio mass spectrometer. The system was tested on a pasture soil from sub-tropical Australia under different soil moisture conditions and combined with 15N tracing in extractable soil N pools to establish a full N balance.ResultsThe method proved to be highly sensitive for detecting N2 (1.12 μg N h−1 kg−1 dry soil (ds)) and N2O (0.36 μg N h−1 kg−1 ds) emissions. The main end product of denitrification in the investigated soil was N2O for both water contents, with N2 accounting for only 3% to 13% of the total denitrification losses. Between 90 and 95% of the added 15N fertiliser could be recovered in N gases and extractable soil N pools.
536 _ _ |a 255 - Terrestrial Systems: From Observation to Prediction (POF3-255)
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700 1 _ |a Meier, Rudolf
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700 1 _ |a Brüggemann, Nicolas
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700 1 _ |a Grace, Peter R.
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700 1 _ |a Dannenmann, Michael
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773 _ _ |a 10.1002/rcm.7689
|g Vol. 30, no. 18, p. 2017 - 2026
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|p 2017 - 2026
|t Rapid communications in mass spectrometry
|v 30
|y 2016
|x 0951-4198
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