TY - JOUR AU - Seyock, Silke AU - Maybeck, Vanessa AU - Offenhäusser, Andreas TI - How to image cell adhesion on soft polymers? JO - Micron VL - 92 SN - 0047-7206 CY - New York, NY [u.a.] PB - Elsevier M1 - FZJ-2016-06439 SP - 39-42 PY - 2017 AB - Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex®) and prepared for electron microscopy. The samples were treated with osmium tetroxide (OsO4) and uranylacetate (UrAc). Best results can be achieved when the polymers were coated with a thin iridium layer before the cell culture. This was done to emphasize the usage of soft polymers as supports for implant electrodes. A good contrast and the adhesion of the cells on soft polymers could be visualized. LB - PUB:(DE-HGF)16 UR - <Go to ISI:>//WOS:000390623600007 C6 - pmid:27866099 DO - DOI:10.1016/j.micron.2016.11.002 UR - https://juser.fz-juelich.de/record/821201 ER -