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001     821201
005     20240619091217.0
024 7 _ |a 10.1016/j.micron.2016.11.002
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100 1 _ |0 P:(DE-Juel1)161234
|a Seyock, Silke
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245 _ _ |a How to image cell adhesion on soft polymers?
260 _ _ |a New York, NY [u.a.]
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520 _ _ |a Here, we present a method to investigate cell adhesion on soft, non-conducting polymers that are implant candidate materials. Neuronal cells were grown on two elastomers (polydimethylsiloxane (PDMS) and Ecoflex®) and prepared for electron microscopy. The samples were treated with osmium tetroxide (OsO4) and uranylacetate (UrAc). Best results can be achieved when the polymers were coated with a thin iridium layer before the cell culture. This was done to emphasize the usage of soft polymers as supports for implant electrodes. A good contrast and the adhesion of the cells on soft polymers could be visualized.
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700 1 _ |0 P:(DE-Juel1)128705
|a Maybeck, Vanessa
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700 1 _ |0 P:(DE-Juel1)128713
|a Offenhäusser, Andreas
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