000823847 001__ 823847
000823847 005__ 20220930130110.0
000823847 0247_ $$2doi$$a10.3390/s16101604
000823847 0247_ $$2Handle$$a2128/12927
000823847 0247_ $$2WOS$$aWOS:000386131600057
000823847 037__ $$aFZJ-2016-06487
000823847 041__ $$aEnglish
000823847 082__ $$a620
000823847 1001_ $$0P:(DE-Juel1)145517$$aSteffen, Victoria$$b0$$ufzj
000823847 245__ $$aA Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid l-Lysine Analysis
000823847 260__ $$aBasel$$bMDPI$$c2016
000823847 3367_ $$2DRIVER$$aarticle
000823847 3367_ $$2DataCite$$aOutput Types/Journal article
000823847 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1479730893_8095
000823847 3367_ $$2BibTeX$$aARTICLE
000823847 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000823847 3367_ $$00$$2EndNote$$aJournal Article
000823847 520__ $$aBackground: The fast development of microbial production strains for basic and fine chemicals is increasingly carried out in small scale cultivation systems to allow for higher throughput. Such parallelized systems create a need for new rapid online detection systems to quantify the respective target compound. In this regard, biosensors, especially genetically encoded Förster resonance energy transfer (FRET)-based biosensors, offer tremendous opportunities. As a proof-of-concept, we have created a toolbox of FRET-based biosensors for the ratiometric determination of L-lysine in fermentation broth. Methods: The sensor toolbox was constructed based on a sensor that consists of an optimized central lysine-/arginine-/ornithine-binding protein (LAO-BP) flanked by two fluorescent proteins (enhanced cyan fluorescent protein (ECFP), Citrine). Further sensor variants with altered affinity and sensitivity were obtained by circular permutation of the binding protein as well as the introduction of flexible and rigid linkers between the fluorescent proteins and the LAO-BP, respectively. Results: The sensor prototype was applied to monitor the extracellular L-lysine concentration of the L-lysine producing Corynebacterium glutamicum (C. glutamicum) strain DM1933 in a BioLector® microscale cultivation device. The results matched well with data obtained by HPLC analysis and the Ninhydrin assay, demonstrating the high potential of FRET-based biosensors for high-throughput microbial bioprocess optimization.
000823847 536__ $$0G:(DE-HGF)POF3-553$$a553 - Physical Basis of Diseases (POF3-553)$$cPOF3-553$$fPOF III$$x0
000823847 588__ $$aDataset connected to CrossRef
000823847 7001_ $$0P:(DE-Juel1)165764$$aOtten, Julia$$b1$$ufzj
000823847 7001_ $$0P:(DE-Juel1)162413$$aEngelmann, Susann$$b2
000823847 7001_ $$0P:(DE-Juel1)158067$$aRadek, Andreas$$b3$$ufzj
000823847 7001_ $$0P:(DE-Juel1)158030$$aLimberg, Michael$$b4$$ufzj
000823847 7001_ $$0P:(DE-Juel1)132009$$aKönig, Bernd$$b5$$ufzj
000823847 7001_ $$0P:(DE-Juel1)129050$$aNoack, Stephan$$b6$$ufzj
000823847 7001_ $$0P:(DE-Juel1)129076$$aWiechert, Wolfgang$$b7$$ufzj
000823847 7001_ $$0P:(DE-Juel1)131522$$aPohl, Martina$$b8$$eCorresponding author$$ufzj
000823847 773__ $$0PERI:(DE-600)2052857-7$$a10.3390/s16101604$$gVol. 16, no. 10, p. 1604 -$$n10$$p1604$$tSensors$$v16$$x1424-8220$$y2016
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.pdf$$yOpenAccess
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.gif?subformat=icon$$xicon$$yOpenAccess
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000823847 8564_ $$uhttps://juser.fz-juelich.de/record/823847/files/A%20Toolbox%20of%20Genetically%20Encoded%20FRET-Based%20Biosensors%20for%20Rapid%20l-Lysine%20Analysis_2016.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000823847 8767_ $$8sensors-146378$$92016-09-20$$d2016-09-20$$eAPC$$jZahlung erfolgt$$psensors-146378$$zCHF 1530,-
000823847 909CO $$ooai:juser.fz-juelich.de:823847$$popenCost$$pVDB$$pdriver$$pOpenAPC$$popen_access$$popenaire$$pdnbdelivery
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)145517$$aForschungszentrum Jülich$$b0$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165764$$aForschungszentrum Jülich$$b1$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)158067$$aForschungszentrum Jülich$$b3$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)158030$$aForschungszentrum Jülich$$b4$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)132009$$aForschungszentrum Jülich$$b5$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129050$$aForschungszentrum Jülich$$b6$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129076$$aForschungszentrum Jülich$$b7$$kFZJ
000823847 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)131522$$aForschungszentrum Jülich$$b8$$kFZJ
000823847 9131_ $$0G:(DE-HGF)POF3-553$$1G:(DE-HGF)POF3-550$$2G:(DE-HGF)POF3-500$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bKey Technologies$$lBioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences$$vPhysical Basis of Diseases$$x0
000823847 9141_ $$y2016
000823847 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000823847 915__ $$0LIC:(DE-HGF)CCBY4$$2HGFVOC$$aCreative Commons Attribution CC BY 4.0
000823847 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000823847 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bSENSORS-BASEL : 2015
000823847 915__ $$0StatID:(DE-HGF)0501$$2StatID$$aDBCoverage$$bDOAJ Seal
000823847 915__ $$0StatID:(DE-HGF)0500$$2StatID$$aDBCoverage$$bDOAJ
000823847 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000823847 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000823847 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000823847 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000823847 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000823847 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000823847 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000823847 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000823847 920__ $$lyes
000823847 9201_ $$0I:(DE-Juel1)ICS-6-20110106$$kICS-6$$lStrukturbiochemie $$x0
000823847 9201_ $$0I:(DE-Juel1)IBG-1-20101118$$kIBG-1$$lBiotechnologie$$x1
000823847 9801_ $$aFullTexts
000823847 980__ $$ajournal
000823847 980__ $$aVDB
000823847 980__ $$aUNRESTRICTED
000823847 980__ $$aI:(DE-Juel1)ICS-6-20110106
000823847 980__ $$aI:(DE-Juel1)IBG-1-20101118
000823847 980__ $$aAPC
000823847 981__ $$aI:(DE-Juel1)IBI-7-20200312
000823847 981__ $$aI:(DE-Juel1)IBG-1-20101118