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000823892 1001_ $$0P:(DE-Juel1)136806$$aKravchenko, Kateryna$$b0
000823892 245__ $$aAnalysis of anticoagulants for blood-based quantitation of amyloid β oligomers in the sFIDA assay
000823892 260__ $$aBerlin [u.a.]$$bde Gruyter$$c2017
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000823892 520__ $$aEarly diagnostics at the preclinical stage of Alzheimer’s disease is of utmost importance for drug development in clinical trials and prognostic guidance. Since soluble Aβ oligomers are considered to play a crucial role in the disease pathogenesis, several methods aim to quantify Aβ oligomers in body fluids such as cerebrospinal fluid (CSF) and blood plasma. The highly specific and sensitive method surface-based fluorescence intensity distribution analysis (sFIDA) has successfully been established for oligomer quantitation in CSF samples. In our study, we explored the sFIDA method for quantitative measurements of synthetic Aβ particles in blood plasma. For this purpose, EDTA, citrate and heparin treated blood plasma samples from five individual donors were spiked with Aβ coated silica nanoparticles (Aβ-SiNaPs) and were applied to the sFIDA assay. Based on the assay parameters linearity, coefficient of variation and limit of detection, we found that EDTA plasma yields the most suitable parameter values for quantitation of Aβ oligomers in sFIDA assay with a limit of detection of 16 fM.
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000823892 7001_ $$0P:(DE-Juel1)162310$$aKulawik, Andreas$$b1$$ufzj
000823892 7001_ $$0P:(DE-Juel1)145968$$aHülsemann, Maren$$b2
000823892 7001_ $$0P:(DE-Juel1)145440$$aKühbach, Katja$$b3$$ufzj
000823892 7001_ $$0P:(DE-Juel1)162137$$aZafiu, Christian$$b4$$ufzj
000823892 7001_ $$0P:(DE-Juel1)159122$$aHerrmann, Yvonne$$b5$$ufzj
000823892 7001_ $$0P:(DE-Juel1)145159$$aLinnartz, Christina$$b6$$ufzj
000823892 7001_ $$0P:(DE-Juel1)145123$$aPeters, Luriano$$b7$$ufzj
000823892 7001_ $$0P:(DE-Juel1)162212$$aBujnicki, Tuyen$$b8$$ufzj
000823892 7001_ $$0P:(DE-Juel1)159192$$aWillbold, Johannes$$b9$$ufzj
000823892 7001_ $$0P:(DE-Juel1)157832$$aBannach, Oliver$$b10$$ufzj
000823892 7001_ $$0P:(DE-Juel1)132029$$aWillbold, Dieter$$b11$$eCorresponding author$$ufzj
000823892 773__ $$0PERI:(DE-600)1466062-3$$a10.1515/hsz-2016-0153$$gVol. 0, no. 0$$n4$$p465–475$$tBiological chemistry$$v398$$x1437-4315$$y2017
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