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@ARTICLE{Herrmann:823896,
      author       = {Herrmann, Yvonne and Kulawik, Andreas and Kühbach, Katja
                      and Hülsemann, Maren and Peters, Luriano and Bujnicki,
                      Tuyen and Kravchenko, Kateryna and Linnartz, Christina and
                      Willbold, Johannes and Zafiu, Christian and Bannach, Oliver
                      and Willbold, Dieter},
      title        = {s{FIDA} automation yields sub-femtomolar limit of detection
                      for {A}β aggregates in body fluids},
      journal      = {Clinical biochemistry},
      volume       = {50},
      number       = {4-5},
      issn         = {0009-9120},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2016-06528},
      pages        = {244-247},
      year         = {2017},
      abstract     = {Objectives: Alzheimer's disease (AD) is a neurodegenerative
                      disorder with yet non-existent therapeutic and limited
                      diagnostic options. Reliable biomarker-based AD diagnostics
                      are of utmost importance for the development and application
                      of therapeutic substances. Wehave previously introduced a
                      platformtechnology designated ‘sFIDA’ for the
                      quantitation of amyloid β peptide (Aβ) aggregates as AD
                      biomarker. In this study we implemented the sFIDA assay on
                      an automated platform to enhance robustness and performance
                      of the assay.Design and methods: In sFIDA (surface-based
                      fluorescence intensity distribution analysis) Aβ species
                      are immobilized by a capture antibody to a glass surface.
                      Aβ aggregates are then multiply loaded with fluorescent
                      antibodies and quantitated by high resolution fluorescence
                      microscopy. As a model system for Aβ aggregates, weused
                      Aβ-conjugated silica nanoparticles (Aβ-SiNaPs) diluted in
                      PBS buffer and cerebrospinal fluid, respectively. Automation
                      of the assay was realized on a liquid handling system in
                      combination with a microplate washer.Results: The automation
                      of the sFIDA assay results in improved intra-assay
                      precision, linearity and sensitivity in comparison to the
                      manual application, and achieved a limit of detection in the
                      sub-femtomolar range.Conclusions: Automation improves the
                      precision and sensitivity of the sFIDA assay, which is a
                      prerequisite for high-throughput measurements and future
                      application of the technology in routine AD diagnostics.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000395965100015},
      pubmed       = {pmid:27823959},
      doi          = {10.1016/j.clinbiochem.2016.11.001},
      url          = {https://juser.fz-juelich.de/record/823896},
}