% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{LeHuu:823900,
      author       = {Le-Huu, Priska and Petrović, Dušan and Strodel, Birgit
                      and Urlacher, Vlada B.},
      title        = {{O}ne-{P}ot, {T}wo-{S}tep {H}ydroxylation of the
                      {M}acrocyclic {D}iterpenoid β-{C}embrenediol {C}atalyzed by
                      {P}450 {BM}3 {M}utants},
      journal      = {ChemCatChem},
      volume       = {8},
      number       = {24},
      issn         = {1867-3880},
      address      = {Weinheim},
      publisher    = {WILEY-VCH Verlag},
      reportid     = {FZJ-2016-06532},
      pages        = {3755-3761},
      year         = {2016},
      abstract     = {Cytochrome P450 monooxygenases (P450s) are involved in the
                      biosynthesis of a wide range of bioactive secondary
                      metabolites. They often introduce several oxy
                      functionalities at different positions of a substrate
                      through multiple steps and produce a range of oxidized
                      derivatives. Herein, we describe a one-pot two-step
                      hydroxylation of the diterpenoid b-cembrenediol isolated
                      from the plant Nicotiana tabacum. This 14-membered
                      macrocycle shows neuroprotective effects and is, along with
                      its oxidized derivatives, of pharmaceutical interest.
                      Sequential hydroxylations catalyzed by the regioselective
                      P450 BM3 mutants F87A/I263L and V78A/F87G yielded the
                      epimeric (9S,10R/S)-b-cembrenetetraols with a diastereomeric
                      ratio of 48:52. The replacement of the mutant V78A/F87G with
                      L75A/ V78A/F87G in the second step improves the
                      diastereomeric ratio up to 10:90. Absolute configurations of
                      the newly introduced hydroxy groups were determined by
                      quantum-mechanical calculations of NMR spectra.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000397015100011},
      doi          = {10.1002/cctc.201600973},
      url          = {https://juser.fz-juelich.de/record/823900},
}