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@INPROCEEDINGS{Buitenhuis:823981,
      author       = {Buitenhuis, Johan and Anop, Hanna},
      title        = {{P}olyelectrolyte complexes from mixtures of oppositely
                      charged filamentous viruses},
      reportid     = {FZJ-2016-06607},
      year         = {2016},
      abstract     = {The fd virus is a filamentous bacteriophage with a length
                      of 880 nm and a diameter of 6.6 nm, that was first isolated
                      from sewage. The virus consists of a single-stranded
                      circular DNA molecule packed in a cylindrical capsid of
                      2,700 identical major coat proteins and a few minor coat
                      proteins at the ends. In the present study the virus is used
                      as a material, i.e. we are not interested in the biological
                      properties, apart from the fact that it can be grown in
                      certain bacteria. Solutions of these viruses can form liquid
                      crystalline phases, are well defined and therefore have been
                      used many times as a model system. By chemical modification
                      of the surface proteins the viruses can be modified, to
                      obtain new properties. An interesting possibility is the
                      modification of the carboxylic groups on the surface of the
                      fd virus by using carbodiimide chemistry to obtain
                      dispersions of charge reversed fd viruses (i.e. positively
                      charged at neutral pH) [1]. As expected, adding dilute
                      solutions of positively charged and negatively charged fd
                      together can result in flocculation. This charge reversal
                      can be combined with a steric stabilization by poly(ethylene
                      glycol) grafting, so that the above mentioned flocculation
                      can be made adjustable and reversible by changing the ionic
                      strength in the solution [1], yielding an interesting model
                      system for the formation of polyelectrolyte complexes (PECs)
                      [2]. Here results on the formation of polyelectrolyte
                      complexes will be presented and the charge of the (modified)
                      viruses is modeled and compared to results from free
                      solution electrophoresis [3].Acknowledgements. We thank M.P.
                      Lettinga and P.R. Lang for useful suggestions[1] Z. Zhang,
                      J. Buitenhuis, A. Cukkemane, M. Brocker, M. Bott and J.K.G.
                      Dhont, Langmuir 2010, 26, 10593.[2] J. van der Gucht, E.
                      Spruijt, M. Lemmers and M.A. Cohen Stuart, J. Colloid
                      Interface Sci. 2011, 361, 407.[3] J. Buitenhuis, Langmuir
                      2012, 28, 13354.},
      month         = {Sep},
      date          = {2016-09-01},
      organization  = {Ostwald Colloquium of the German
                       Colloid Society, RWTH Aachen (Germany),
                       1 Sep 2016 - 2 Sep 2016},
      subtyp        = {After Call},
      cin          = {ICS-3},
      cid          = {I:(DE-Juel1)ICS-3-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/823981},
}