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@ARTICLE{Bucciarelli:824671,
author = {Bucciarelli, S. and Myung, J. S. and Farago, B. and Das, S.
and Vliegenthart, Gerrit and Holderer, O. and Winkler, R. G.
and Schurtenberger, P. and Gompper, G. and Stradner, A.},
title = {{D}ramatic influence of patchy attractions on short-time
protein diffusion under crowded conditions},
journal = {Science advances},
volume = {2},
number = {12},
issn = {2375-2548},
address = {Washington, DC [u.a.]},
publisher = {Assoc.},
reportid = {FZJ-2016-07229},
pages = {e1601432 - e1601432},
year = {2016},
abstract = {In the dense and crowded environment of the cell cytoplasm,
an individual protein feels the presence of and interacts
with all surrounding proteins. While we expect this to
strongly influence the short-time diffusion coefficient Ds
of proteins on length scales comparable to the
nearest-neighbor distance, this quantity is difficult to
assess experimentally. We demonstrate that quantitative
information about Ds can be obtained from quasi-elastic
neutron scattering experiments using the neutron spin echo
technique. We choose two well-characterized and highly
stable eye lens proteins, bovine α-crystallin and
γB-crystallin, and measure their diffusion at
concentrations comparable to those present in the eye lens.
While diffusion slows down with increasing concentration for
both proteins, we find marked variations that are directly
linked to subtle differences in their interaction
potentials. A comparison with computer simulations shows
that anisotropic and patchy interactions play an essential
role in determining the local short-time dynamics. Hence,
our study clearly demonstrates the enormous effect that weak
attractions can have on the short-time diffusion of proteins
at concentrations comparable to those in the cellular
cytosol.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / ICS-2},
ddc = {500},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)ICS-2-20110106},
pnm = {6215 - Soft Matter, Health and Life Sciences (POF3-621) /
6G15 - FRM II / MLZ (POF3-6G15) / 551 - Functional
Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-6215 / G:(DE-HGF)POF3-6G15 /
G:(DE-HGF)POF3-551},
experiment = {EXP:(DE-MLZ)J-NSE-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000391268800014},
pubmed = {pmid:27957539},
doi = {10.1126/sciadv.1601432},
url = {https://juser.fz-juelich.de/record/824671},
}