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@ARTICLE{Hemmerich:824717,
      author       = {Hemmerich, Johannes and Rohe, Peter and Kleine, Britta and
                      Jurischka, Sarah-Kristin and Wiechert, Wolfgang and Freudl,
                      Roland and Oldiges, Marco},
      title        = {{U}se of a {S}ec signal peptide library from {B}acillus
                      subtilis for the optimization of cutinase secretion in
                      {C}orynebacterium glutamicum},
      journal      = {Microbial cell factories},
      volume       = {15},
      number       = {1},
      issn         = {1475-2859},
      address      = {London},
      publisher    = {Biomed Central},
      reportid     = {FZJ-2016-07275},
      pages        = {208},
      year         = {2016},
      abstract     = {Technical bulk enzymes represent a huge market, and the
                      extracellular production of such enzymes is favorable due to
                      lowered cost for product recovery. Protein secretion can be
                      achieved via general secretion (Sec) pathway. Specific
                      sequences, signal peptides (SPs), are necessary to direct
                      the target protein into the translocation machinery. For
                      example, >150 Sec-specific SPs have been identified for
                      Bacillus subtilis alone. As the best SP for a target protein
                      of choice cannot be predicted a priori, screening of
                      homologous SPs has been shown to be a powerful tool for
                      different expression organisms. While SP libraries between
                      closely related species were successfully applied to
                      optimize recombinant protein secretion, this was not
                      investigated for distantly related species. Therefore, in
                      this study a Sec SP library from low-GC firmicutes B.
                      subtilis is investigated to optimize protein secretion in
                      high-GC actinobacterium Corynebacterium glutamicum using
                      cutinase from Fusarium solani pisi as model protein. [...]},
      cin          = {IBG-1},
      ddc          = {610},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000390285500002},
      doi          = {10.1186/s12934-016-0604-6},
      url          = {https://juser.fz-juelich.de/record/824717},
}