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@ARTICLE{Stingaciu:824954,
author = {Stingaciu, Laura and O’Neill, Hugh and Liberton, Michelle
and Urban, Volker S. and Pakrasi, Himadri B. and Ohl,
Michael},
title = {{R}evealing the {D}ynamics of {T}hylakoid {M}embranes in
{L}iving {C}yanobacterial {C}ells},
journal = {Scientific reports},
volume = {6},
issn = {2045-2322},
address = {London},
publisher = {Nature Publishing Group},
reportid = {FZJ-2016-07450},
pages = {19627},
year = {2016},
abstract = {Cyanobacteria are photosynthetic prokaryotes that make
major contributions to the production of the oxygen in the
Earth atmosphere. The photosynthetic machinery in
cyanobacterial cells is housed in flattened membrane
structures called thylakoids. The structural organization of
cyanobacterial cells and the arrangement of the thylakoid
membranes in response to environmental conditions have been
widely investigated. However, there is limited knowledge
about the internal dynamics of these membranes in terms of
their flexibility and motion during the photosynthetic
process. We present a direct observation of thylakoid
membrane undulatory motion in vivo and show a connection
between membrane mobility and photosynthetic activity.
High-resolution inelastic neutron scattering experiments on
the cyanobacterium Synechocystis sp. PCC 6803 assessed the
flexibility of cyanobacterial thylakoid membrane sheets and
the dependence of the membranes on illumination conditions.
We observed softer thylakoid membranes in the dark that have
three-to four fold excess mobility compared to membranes
under high light conditions. Our analysis indicates that
electron transfer between photosynthetic reaction centers
and the associated electrochemical proton gradient across
the thylakoid membrane result in a significant driving force
for excess membrane dynamics. These observations provide a
deeper understanding of the relationship between
photosynthesis and cellular architecture.},
cin = {ICS-1 / Neutronenstreuung ; JCNS-1 / JCNS-SNS},
ddc = {000},
cid = {I:(DE-Juel1)ICS-1-20110106 / I:(DE-Juel1)JCNS-1-20110106 /
I:(DE-Juel1)JCNS-SNS-20110128},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551) /
6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
/ 6215 - Soft Matter, Health and Life Sciences (POF3-621)},
pid = {G:(DE-HGF)POF3-551 / G:(DE-HGF)POF3-6G4 /
G:(DE-HGF)POF3-6215},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000368778100001},
pubmed = {pmid:26790980},
doi = {10.1038/srep19627},
url = {https://juser.fz-juelich.de/record/824954},
}