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@ARTICLE{Gupta:824977,
author = {Gupta, Sudipta and Biehl, Ralf and Sill, Clemens and
Allgaier, J. and Sharp, Melissa and Ohl, Michael and
Richter, Dieter},
title = {{P}rotein {E}ntrapment in {P}olymeric {M}esh: {D}iffusion
in {C}rowded {E}nvironment with {F}ast {P}rocess on {S}hort
{S}cales},
journal = {Macromolecules},
volume = {49},
number = {5},
issn = {1520-5835},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2016-07473},
pages = {1941 - 1949},
year = {2016},
abstract = {The natural environment of proteins is a crowded
environment as in cells, extracellular fluids, or during
processing. Semidilute polymer solutions have been a source
of rich structural and dynamical properties and mimic a
crowded environment, but a proper understanding of protein
dynamics in the crowded environment is far lagging. Such a
study not only realizes protein’s natural environment in a
crowded solution in the cell or during processing but also
manifests the underlying protein–polymer interaction. By
dispersing model globular proteins like α-lactalbumin (La)
and hemoglobin (Hb), in aqueous solution of poly(ethylene
oxide) (PEO) we mimic a crowded environment and use
state-of-the-art neutron spin echo (NSE) and small-angle
neutron scattering (SANS) techniques to observe the
corresponding protein dynamics in semidilute polymer
solution. NSE can access the fast diffusion process (Dfast)
prior to the slow diffusion process on long times and length
scales (Dγ). The protein dynamics in a crowded environment
can be described analogous to the diffusion in a periodic
potential. The fast dynamics corresponds to diffusion inside
a trap built by the polymer mesh while the slower process is
the long time diffusion on macroscopic length scales also
observed by other techniques. We observe the onset of
fractional diffusion for higher concentrated polymer
solutions.},
cin = {ICS-1 / Neutronenstreuung ; JCNS-1 / JCNS-SNS / JCNS-2},
ddc = {540},
cid = {I:(DE-Juel1)ICS-1-20110106 / I:(DE-Juel1)JCNS-1-20110106 /
I:(DE-Juel1)JCNS-SNS-20110128 / I:(DE-Juel1)JCNS-2-20110106},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551) /
6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623)
/ 6215 - Soft Matter, Health and Life Sciences (POF3-621)},
pid = {G:(DE-HGF)POF3-551 / G:(DE-HGF)POF3-6G4 /
G:(DE-HGF)POF3-6215},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000371851100047},
doi = {10.1021/acs.macromol.5b02281},
url = {https://juser.fz-juelich.de/record/824977},
}