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@ARTICLE{Junker:825478,
      author       = {Junker, Laura and Ensminger, Ingo},
      title        = {{F}ast detection of leaf pigments and isoprenoids for
                      ecophysiological studies, plant phenotyping and validating
                      remote-sensing of vegetation},
      journal      = {Physiologia plantarum},
      volume       = {158},
      number       = {4},
      issn         = {0031-9317},
      address      = {Oxford [u.a.]},
      publisher    = {Wiley-Blackwell},
      reportid     = {FZJ-2016-07941},
      pages        = {369 - 381},
      year         = {2016},
      abstract     = {Rapid developments in remote-sensing of vegetation and
                      high-throughput precision plant phenotyping promise a range
                      of real-life applications using leaf optical properties for
                      non-destructive assessment of plant performance. Use of leaf
                      optical properties for assessing plant performance requires
                      the ability to use photosynthetic pigments as proxies for
                      physiological properties and the ability to detect these
                      pigments fast, reliably and at low cost. We describe a
                      simple and cost-effective protocol for the rapid analysis of
                      chlorophylls, carotenoids and tocopherols using
                      high-performance liquid chromatography (HPLC). Many existing
                      methods are based on the expensive solvent acetonitrile,
                      take a long time or do not include lutein epoxide and
                      α-carotene. We aimed to develop an HPLC method which
                      separates all major chlorophylls and carotenoids as well as
                      lutein epoxide, α-carotene and α-tocopherol. Using a
                      C30-column and a mobile phase with a gradient of methanol,
                      methyl-tert-butyl-ether (MTBE) and water, our method
                      separates the above pigments and isoprenoids within 28 min.
                      The broad applicability of our method is demonstrated using
                      samples from various plant species and tissue types, e.g.
                      leaves of Arabidopsis and avocado plants, several deciduous
                      and conifer tree species, various crops, stems of parasitic
                      dodder, fruit of tomato, roots of carrots and Chlorella
                      algae. In comparison to previous methods, our method is very
                      affordable, fast and versatile and can be used to analyze
                      all major photosynthetic pigments that contribute to changes
                      in leaf optical properties and which are of interest in most
                      ecophysiological studies.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000387869400001},
      pubmed       = {pmid:27616618},
      doi          = {10.1111/ppl.12512},
      url          = {https://juser.fz-juelich.de/record/825478},
}