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@ARTICLE{Wachtmeister:826010,
      author       = {Wachtmeister, Jochen and Jakoblinnert, Andre and Rother,
                      Dörte},
      title        = {{S}tereoselective {T}wo-{S}tep {B}iocatalysis in {O}rganic
                      {S}olvent: {T}oward {A}ll {S}tereoisomers of a 1,2-{D}iol at
                      {H}igh {P}roduct {C}oncentrations},
      journal      = {Organic process research $\&$ development},
      volume       = {20},
      number       = {10},
      issn         = {1520-586X},
      address      = {Washington, DC},
      publisher    = {ACS Publ.},
      reportid     = {FZJ-2017-00282},
      pages        = {1744 - 1753},
      year         = {2016},
      abstract     = {Biotransformations on larger scale are mostly limited to
                      cases in which alternative chemical routes lack sufficient
                      chemo-, regio-, or stereoselectivity. Here, we expand the
                      applicability of biocatalysis by combining cheap whole cell
                      catalysts with a microaqueous solvent system. Compared to
                      aqueous systems, this permits manifoldly higher
                      concentrations of hydrophobic substrates while maintaining
                      stereoselectivity. We apply these methods to four different
                      two-step reactions of carboligation and oxidoreduction to
                      obtain 1-phenylpropane-1,2-diol (PPD), a versatile building
                      block for pharmaceuticals, starting from inexpensive
                      aldehyde substrates. By a modular combination of two
                      carboligases and two alcohol dehydrogenases, all four
                      stereoisomers of PPD can be produced in a flexible way.
                      After thorough optimization of each two-step reaction, the
                      resulting processes enabled up to 63 g L–1 product
                      concentration $(98\%$ yield), space-time-yields up to 144 g
                      L–1 d–1, and a target isomer content of at least $95\%.$
                      Despite the use of whole cell catalysts, we did not observe
                      any side product formation of note. In addition, we prove
                      that, by using 1,5-pentandiol as a smart cosubstrate, a very
                      advantageous cofactor regeneration system could be applied.},
      cin          = {IBG-1},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000386188400007},
      doi          = {10.1021/acs.oprd.6b00232},
      url          = {https://juser.fz-juelich.de/record/826010},
}