Gast ::
| Hauptseite > Publikationsdatenbank > Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme > print |
| Hauptseite > Publikationsdatenbank > Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme > print |
| 001 | 826420 | ||
| 005 | 20210129225615.0 | ||
| 024 | 7 | _ | |a 10.1038/ncomms13445 |2 doi |
| 024 | 7 | _ | |a 2128/13579 |2 Handle |
| 024 | 7 | _ | |a WOS:000388990900001 |2 WOS |
| 024 | 7 | _ | |a altmetric:14154665 |2 altmetric |
| 024 | 7 | _ | |a pmid:27897163 |2 pmid |
| 037 | _ | _ | |a FZJ-2017-00648 |
| 082 | _ | _ | |a 500 |
| 100 | 1 | _ | |a Kwon, Hanna |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme |
| 260 | _ | _ | |a London |c 2016 |b Nature Publishing Group |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1485253759_15330 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Catalytic heme enzymes carry out a wide range of oxidations in biology. They have in common a mechanism that requires formation of highly oxidized ferryl intermediates. It is these ferryl intermediates that provide the catalytic engine to drive the biological activity. Unravelling the nature of the ferryl species is of fundamental and widespread importance. The essential question is whether the ferryl is best described as a Fe(IV)=O or a Fe(IV)–OH species, but previous spectroscopic and X-ray crystallographic studies have not been able to unambiguously differentiate between the two species. Here we use a different approach. We report a neutron crystal structure of the ferryl intermediate in Compound II of a heme peroxidase; the structure allows the protonation states of the ferryl heme to be directly observed. This, together with pre-steady state kinetic analyses, electron paramagnetic resonance spectroscopy and single crystal X-ray fluorescence, identifies a Fe(IV)–OH species as the reactive intermediate. The structure establishes a precedent for the formation of Fe(IV)–OH in a peroxidase. |
| 536 | _ | _ | |0 G:(DE-HGF)POF3-6G15 |f POF III |x 0 |c POF3-6G15 |a 6G15 - FRM II / MLZ (POF3-6G15) |
| 536 | _ | _ | |a 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) |0 G:(DE-HGF)POF3-6G4 |c POF3-623 |f POF III |x 1 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 650 | 2 | 7 | |a Biology |0 V:(DE-MLZ)SciArea-160 |2 V:(DE-HGF) |x 0 |
| 650 | 1 | 7 | |a Health and Life |0 V:(DE-MLZ)GC-130-2016 |2 V:(DE-HGF) |x 0 |
| 693 | _ | _ | |a Forschungs-Neutronenquelle Heinz Maier-Leibnitz |e BIODIFF: Diffractometer for large unit cells |f NL1 |1 EXP:(DE-MLZ)FRMII-20140101 |0 EXP:(DE-MLZ)BIODIFF-20140101 |5 EXP:(DE-MLZ)BIODIFF-20140101 |6 EXP:(DE-MLZ)NL1-20140101 |x 0 |
| 700 | 1 | _ | |a Basran, Jaswir |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Casadei, Cecilia M. |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Fielding, Alistair J. |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Schrader, Tobias E. |0 P:(DE-Juel1)138266 |b 4 |
| 700 | 1 | _ | |a Ostermann, Andreas |0 P:(DE-HGF)0 |b 5 |
| 700 | 1 | _ | |a Devos, Juliette M. |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Aller, Pierre |0 P:(DE-HGF)0 |b 7 |
| 700 | 1 | _ | |a Blakeley, Matthew P. |0 0000-0002-6412-4358 |b 8 |
| 700 | 1 | _ | |a Moody, Peter C. E. |0 P:(DE-HGF)0 |b 9 |e Corresponding author |
| 700 | 1 | _ | |a Raven, Emma L. |0 P:(DE-HGF)0 |b 10 |e Corresponding author |
| 773 | _ | _ | |a 10.1038/ncomms13445 |g Vol. 7, p. 13445 - |0 PERI:(DE-600)2553671-0 |p 13445 - |t Nature Communications |v 7 |y 2016 |x 2041-1723 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.pdf |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.gif?subformat=icon |x icon |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.jpg?subformat=icon-1440 |x icon-1440 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.jpg?subformat=icon-180 |x icon-180 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.jpg?subformat=icon-640 |x icon-640 |y OpenAccess |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/826420/files/ncomms13445-1.pdf?subformat=pdfa |x pdfa |y OpenAccess |
| 909 | C | O | |o oai:juser.fz-juelich.de:826420 |p openaire |p VDB:MLZ |p driver |p open_access |p VDB |p dnbdelivery |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)138266 |
| 913 | 1 | _ | |a DE-HGF |9 G:(DE-HGF)POF3-6G15 |x 0 |4 G:(DE-HGF)POF |v FRM II / MLZ |1 G:(DE-HGF)POF3-6G0 |0 G:(DE-HGF)POF3-6G15 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-600 |b Forschungsbereich Materie |l Großgeräte: Materie |
| 913 | 1 | _ | |a DE-HGF |b Forschungsbereich Materie |l Von Materie zu Materialien und Leben |1 G:(DE-HGF)POF3-620 |0 G:(DE-HGF)POF3-623 |2 G:(DE-HGF)POF3-600 |v Facility topic: Neutrons for Research on Condensed Matter |9 G:(DE-HGF)POF3-6G4 |x 1 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2016 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a Creative Commons Attribution CC BY 4.0 |0 LIC:(DE-HGF)CCBY4 |2 HGFVOC |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1040 |2 StatID |b Zoological Record |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT COMMUN : 2015 |
| 915 | _ | _ | |a IF >= 10 |0 StatID:(DE-HGF)9910 |2 StatID |b NAT COMMUN : 2015 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a OpenAccess |0 StatID:(DE-HGF)0510 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1150 |2 StatID |b Current Contents - Physical, Chemical and Earth Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-FRM-II-20110218 |k JCNS (München) ; Jülich Centre for Neutron Science JCNS (München) ; JCNS-FRM-II |l JCNS-FRM-II |x 0 |
| 920 | 1 | _ | |0 I:(DE-Juel1)JCNS-1-20110106 |k Neutronenstreuung ; JCNS-1 |l Neutronenstreuung |x 1 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a UNRESTRICTED |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-FRM-II-20110218 |
| 980 | _ | _ | |a I:(DE-Juel1)JCNS-1-20110106 |
| 980 | 1 | _ | |a FullTexts |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|