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@ARTICLE{Wang:826554,
      author       = {Wang, Wei and ma, peixiang and Dong, Hui and Krause,
                      Hans-Joachim and Zhang, Yi and Willbold, Dieter and
                      Offenhäusser, Andreas and Gu, Zhongwei},
      title        = {{A} magnetic nanoparticles relaxation sensor for
                      protein-protein interaction detection at ultra-low magnetic
                      field},
      journal      = {Biosensors and bioelectronics},
      volume       = {80},
      issn         = {0956-5663},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2017-00773},
      pages        = {661–665},
      year         = {2016},
      abstract     = {Functionalized magnetic nanoparticles (MNPs) can serve as
                      magnetic relaxation sensors (MRSs) to detect different
                      biological targets, because the clustering of magnetic
                      particle may cause the spin-spin relaxation time (T2)
                      decrease of the surrounding water protons. However, the
                      application of MNPs in clinical NMR systems faces the
                      challenge of poor stability at magnetic field strengths in
                      the order of tesla. The recently developed ultra-low field
                      (ULF) NMR technique working at microtesla (μT) range then
                      becomes a candidate. Herein, we incorporated superconducting
                      quantum interference device (SQUID) as the detector in the
                      ultra-low field system to enhance the sensitivity. We
                      functionalized the Fe3O4 nanoparticles with the
                      gama-aminobutyrate type A receptor-associated proteins
                      (GABARAP), which specifically interact with calreticulin
                      (CRT). As a result of the interaction between GABARAP and
                      CRT, the clustering of the functionalized MNPs generates
                      local magnetic fields, which accelerate the dephasing of the
                      water protons in the vicinity. We analyzed the relation
                      between T2 values and the CRT concentrations at 211μT and
                      the low detection limit for CRT is 10 pg/ml, which is
                      superior to the immunoblot system. The high sensitivity of
                      the ULF NMR system for protein-protein interaction detection
                      demonstrates the potential to use this inexpensive, portable
                      system for quick biochemical and clinical assays.},
      cin          = {ICS-6},
      ddc          = {570},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000372558500091},
      doi          = {10.1016/j.bios.2016.02.037},
      url          = {https://juser.fz-juelich.de/record/826554},
}