TY  - JOUR
AU  - Herrmann, Yvonne
AU  - Bujnicki, Tuyen
AU  - Zafiu, Christian
AU  - Kulawik, Andreas
AU  - Kühbach, Katja
AU  - Peters, Luriano
AU  - Fabig, Judith
AU  - Willbold, Johannes
AU  - Bannach, Oliver
AU  - Willbold, Dieter
TI  - Nanoparticle standards for immuno-based quantitation of α-synuclein oligomers in diagnostics of Parkinson's disease and other synucleopathies
JO  - Clinica chimica acta
VL  - 466
SN  - 0009-8981
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2017-00777
SP  - 152–159
PY  - 2017
AB  - Parkinson's disease is a neurodegenerative disorder that is characterized by symptoms such as rigor, tremor and bradykinesia. A reliable and early diagnosis could improve the development of early therapeutic strategies before death of dopaminergic neurons leads to the first clinical symptoms.The sFIDA (surface-based fluorescence intensity distribution analysis) assay is a highly sensitive method to determine the concentration of α-synuclein (α-syn) oligomers, which are presumably the major toxic isoform of α-syn and potentially the most direct biomarker for PD.Oligomer-based diagnostic tests require standard molecules that closely mimic the native oligomer. This is particularly important for calibration and assessment of inter-assay variation. In this study, we generated a standard in form of α-syn coated silica nanoparticles (α-syn-SiNaPs) that are in the size range of α-syn oligomers and provide a defined number of α-syn epitopes.The preparation of the sFIDA assay was realized on an automated platform to allow handling of high number of samples and reduce the effects of human error. The assay outcome was analyzed by determination of coefficient of variation and linearity for the applied α-syn-SiNaPs concentrations. Additionally, the limit of detection and lower limit of quantification were determined yielding concentrations in the lower femtomolar range.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000397834600024
C6  - pmid:28088342
DO  - DOI:10.1016/j.cca.2017.01.010
UR  - https://juser.fz-juelich.de/record/826558
ER  -