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@ARTICLE{Herrmann:826558,
      author       = {Herrmann, Yvonne and Bujnicki, Tuyen and Zafiu, Christian
                      and Kulawik, Andreas and Kühbach, Katja and Peters, Luriano
                      and Fabig, Judith and Willbold, Johannes and Bannach, Oliver
                      and Willbold, Dieter},
      title        = {{N}anoparticle standards for immuno-based quantitation of
                      α-synuclein oligomers in diagnostics of {P}arkinson's
                      disease and other synucleopathies},
      journal      = {Clinica chimica acta},
      volume       = {466},
      issn         = {0009-8981},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2017-00777},
      pages        = {152–159},
      year         = {2017},
      abstract     = {Parkinson's disease is a neurodegenerative disorder that is
                      characterized by symptoms such as rigor, tremor and
                      bradykinesia. A reliable and early diagnosis could improve
                      the development of early therapeutic strategies before death
                      of dopaminergic neurons leads to the first clinical
                      symptoms.The sFIDA (surface-based fluorescence intensity
                      distribution analysis) assay is a highly sensitive method to
                      determine the concentration of α-synuclein (α-syn)
                      oligomers, which are presumably the major toxic isoform of
                      α-syn and potentially the most direct biomarker for
                      PD.Oligomer-based diagnostic tests require standard
                      molecules that closely mimic the native oligomer. This is
                      particularly important for calibration and assessment of
                      inter-assay variation. In this study, we generated a
                      standard in form of α-syn coated silica nanoparticles
                      (α-syn-SiNaPs) that are in the size range of α-syn
                      oligomers and provide a defined number of α-syn
                      epitopes.The preparation of the sFIDA assay was realized on
                      an automated platform to allow handling of high number of
                      samples and reduce the effects of human error. The assay
                      outcome was analyzed by determination of coefficient of
                      variation and linearity for the applied α-syn-SiNaPs
                      concentrations. Additionally, the limit of detection and
                      lower limit of quantification were determined yielding
                      concentrations in the lower femtomolar range.},
      cin          = {ICS-6},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000397834600024},
      pubmed       = {pmid:28088342},
      doi          = {10.1016/j.cca.2017.01.010},
      url          = {https://juser.fz-juelich.de/record/826558},
}