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@INPROCEEDINGS{Junker:826755,
      author       = {Junker, Laura and Thiele, Björn and Wormit, Alexandra and
                      Usadel, Björn and Schurr, Ulrich and Wiese-Klinkenberg,
                      Anika},
      title        = {{I}nduc{T}om{E} – {I}nduction of secondary metabolites in
                      tomato leaves},
      reportid     = {FZJ-2017-00973},
      year         = {2016},
      abstract     = {Plant secondary metabolites are essential components of the
                      human diet, serve as high-value fine chemicals and are
                      utilized as phytomedicines and industrial raw materials.
                      Chemically, secondary metabolites exhibit an enormous
                      diversity and complexity which makes their industrial
                      chemical synthesis difficult and expensive. Therefore, they
                      are often extracted from plants which are grown especially
                      for this purpose, like medicinal plants. On the other hand,
                      large quantities of plant biomass are produced as
                      by-products of horticultural food crop production. As plants
                      increase the production of secondary metabolites in response
                      to abiotic stress, post-harvest treatments of
                      greenhouse-grown crop plants might induce the accumulation
                      of secondary metabolites in these by-products. The
                      identification of suitable stress treatments and extraction
                      procedures would enhance the concentration of secondary
                      metabolites in the green biomass of commercially grown crops
                      and allow for a profitable extraction. The utilization of
                      such by-products for extraction of secondary metabolites
                      would thus add extra value to horticultural crop plant
                      production.The project InducTomE
                      (http://www.biosc.de/inductome) studies the induction of
                      secondary metabolites, namely solanesol and rutin, in tomato
                      by-products, and develops a conceptual process design
                      including extraction procedures and evaluation of the
                      emerging value chains and their economic feasibility. As
                      part of the project, our group evaluates the impact of
                      abiotic stress treatments on the accumulation of secondary
                      metabolites in tomato leaves. We aim to identify abiotic
                      stress conditions which maximize the induction of these
                      target metabolites and can also be cost-efficiently applied
                      to commercially grown mature tomato plants.One-month-old
                      plants of different lines of commercially grown tomato lines
                      in comparison to their wild ancestor, Solanum pennellii, are
                      subjected to different abiotic stress treatments in climate
                      chambers. The content analysis of solanesol and rutin by
                      liquid chromatography-mass spectrometry (LC-MS) is combined
                      with qPCR analyses of key genes involved in their
                      biosynthetic pathways. In addition, plant growth and stress
                      responses are captured by using plant phenotyping imaging
                      techniques. First experiments compared the response of the
                      commercially used tomato line Lyterno and the wild S.
                      pennellii to nitrogen deficiency and drought. Leaf samples
                      were analysed after stress and recovery from nitrogen
                      deficiency and drought. Further experiments will also
                      include cold and light treatments and combinations of
                      nitrogen deficiency, drought, cold and light treatments.
                      When the most suitable abiotic stress treatment is
                      identified, the biosynthetic pathways for solanesol and
                      rutin will be investigated, and plants will be screened for
                      further secondary metabolites which might be of interest for
                      commercial applications.Based on our results, our project
                      partners will apply the most suitable stress treatment to
                      greenhouse grown tomato plants, in order to optimize yield
                      in plants grown under conditions used in horticultural food
                      crop production. Process engineers will develop a technical
                      separation process for the extraction of the target
                      metabolites from green tomato residues. Moreover, the
                      production and capital costs of the downstream processing
                      and the emerging value chains and market potentials will be
                      analysed.},
      month         = {Sep},
      date          = {2016-09-11},
      organization  = {DBG-Sektionstagung Pflanzliche
                       Naturstoffe, Meisdorf (Germany), 11 Sep
                       2016 - 13 Sep 2016},
      subtyp        = {After Call},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/826755},
}