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| Hauptseite > Publikationsdatenbank > InducTomE – Induction of secondary metabolites in tomato leaves |
| Hauptseite > Publikationsdatenbank > InducTomE – Induction of secondary metabolites in tomato leaves |
| Conference Presentation (After Call) | FZJ-2017-00973 |
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2016
Abstract: Plant secondary metabolites are essential components of the human diet, serve as high-value fine chemicals and are utilized as phytomedicines and industrial raw materials. Chemically, secondary metabolites exhibit an enormous diversity and complexity which makes their industrial chemical synthesis difficult and expensive. Therefore, they are often extracted from plants which are grown especially for this purpose, like medicinal plants. On the other hand, large quantities of plant biomass are produced as by-products of horticultural food crop production. As plants increase the production of secondary metabolites in response to abiotic stress, post-harvest treatments of greenhouse-grown crop plants might induce the accumulation of secondary metabolites in these by-products. The identification of suitable stress treatments and extraction procedures would enhance the concentration of secondary metabolites in the green biomass of commercially grown crops and allow for a profitable extraction. The utilization of such by-products for extraction of secondary metabolites would thus add extra value to horticultural crop plant production.The project InducTomE (http://www.biosc.de/inductome) studies the induction of secondary metabolites, namely solanesol and rutin, in tomato by-products, and develops a conceptual process design including extraction procedures and evaluation of the emerging value chains and their economic feasibility. As part of the project, our group evaluates the impact of abiotic stress treatments on the accumulation of secondary metabolites in tomato leaves. We aim to identify abiotic stress conditions which maximize the induction of these target metabolites and can also be cost-efficiently applied to commercially grown mature tomato plants.One-month-old plants of different lines of commercially grown tomato lines in comparison to their wild ancestor, Solanum pennellii, are subjected to different abiotic stress treatments in climate chambers. The content analysis of solanesol and rutin by liquid chromatography-mass spectrometry (LC-MS) is combined with qPCR analyses of key genes involved in their biosynthetic pathways. In addition, plant growth and stress responses are captured by using plant phenotyping imaging techniques. First experiments compared the response of the commercially used tomato line Lyterno and the wild S. pennellii to nitrogen deficiency and drought. Leaf samples were analysed after stress and recovery from nitrogen deficiency and drought. Further experiments will also include cold and light treatments and combinations of nitrogen deficiency, drought, cold and light treatments. When the most suitable abiotic stress treatment is identified, the biosynthetic pathways for solanesol and rutin will be investigated, and plants will be screened for further secondary metabolites which might be of interest for commercial applications.Based on our results, our project partners will apply the most suitable stress treatment to greenhouse grown tomato plants, in order to optimize yield in plants grown under conditions used in horticultural food crop production. Process engineers will develop a technical separation process for the extraction of the target metabolites from green tomato residues. Moreover, the production and capital costs of the downstream processing and the emerging value chains and market potentials will be analysed.
Keyword(s): Biology (2nd)
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