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@ARTICLE{Ghaffari:826913,
author = {Ghaffari, Mohammad R. and Shahinnia, Fahimeh and Usadel,
Björn and Junker, Björn and Schreiber, Falk and
Sreenivasulu, Nese and Hajirezaei, Mohammad R.},
title = {{T}he {M}etabolic {S}ignature of {B}iomass {F}ormation in
{B}arley},
journal = {Plant $\&$ cell physiology},
volume = {57},
number = {9},
issn = {1471-9053},
address = {Oxford},
publisher = {Oxford University Press},
reportid = {FZJ-2017-01129},
pages = {1943 - 1960},
year = {2016},
abstract = {The network analysis of genome-wide transcriptome
responses, metabolic signatures and enzymes’ relationship
to biomass formation has been studied in a diverse panel of
12 barley accessions during vegetative and reproductive
stages. The primary metabolites and enzymes involved in
central metabolism that determine the accumulation of shoot
biomass at the vegetative stage of barley development are
primarily being linked to sucrose accumulation and sucrose
synthase activity. Interestingly, the metabolic and enzyme
links which are strongly associated with biomass
accumulation during reproductive stages are related to
starch accumulation and tricarboxylic acid (TCA) cycle
intermediates citrate, malate, trans -aconitate and
isocitrate. Additional significant associations were also
found for UDP glucose, ATP and the amino acids isoleucine,
valine, glutamate and histidine during the reproductive
stage. A network analysis resulted in a combined
identification of metabolite and enzyme signatures
indicative for grain weight accumulation that was correlated
with the activity of ADP-glucose pyrophosphorylase (AGPase),
a rate-limiting enzyme involved in starch biosynthesis, and
with that of alanine amino transferase involved in the
synthesis of storage proteins. We propose that the mechanism
related to vegetative and reproductive biomass formation vs.
seed biomass formation is being linked to distinct fluxes
regulating sucrose, starch, sugars and amino acids as
central resources. These distinct biomarkers can be used to
engineer biomass production and grain weight in barley.},
cin = {IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-2-20101118},
pnm = {582 - Plant Science (POF3-582)},
pid = {G:(DE-HGF)POF3-582},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000384717400013},
pubmed = {pmid:27388338},
doi = {10.1093/pcp/pcw117},
url = {https://juser.fz-juelich.de/record/826913},
}