TY  - JOUR
AU  - Streich, Carmen
AU  - Akkari, Laura
AU  - Decker, Christina
AU  - Bormann, Jenny
AU  - Rehbock, Christoph
AU  - Müller-Schiffmann, Andreas
AU  - Niemeyer, Felix Carlsson
AU  - Nagel-Steger, Luitgard
AU  - Willbold, Dieter
AU  - Sacca, Barbara
AU  - Korth, Carsten
AU  - Schrader, Thomas
AU  - Barcikowski, Stephan
TI  - Characterizing the Effect of Multivalent Conjugates Composed of Aβ-Specific Ligands and Metal Nanoparticles on Neurotoxic Fibrillar Aggregation
JO  - ACS nano
VL  - 10
IS  - 8
SN  - 1936-0851
CY  - Washington, DC
PB  - Soc.
M1  - FZJ-2017-01157
SP  - 7582-7597
PY  - 2016
AB  - Therapeutically active small molecules represent promising nonimmunogenic alternatives to antibodies for specifically targeting disease-relevant receptors. However, a potential drawback compared to antibody–antigen interactions may be the lower affinity of small molecules toward receptors. Here, we overcome this low-affinity problem by coating the surface of nanoparticles (NPs) with multiple ligands. Specifically, we explored the use of gold and platinum nanoparticles to increase the binding affinity of Aβ-specific small molecules to inhibit Aβ peptide aggregation into fibrils in vitro. The interactions of bare NPs, free ligands, and NP-bound ligands with Aβ are comprehensively studied via physicochemical methods (spectroscopy, microscopy, immunologic tests) and cell assays. Reduction of thioflavin T fluorescence, as an indicator for β-sheet content, and inhibition of cellular Aβ excretion are even more effective with NP-bound ligands than with the free ligands. The results from this study may have implications in the development of therapeutics for treating Alzheimer’s disease.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000381959100041
C6  - pmid:27404114
DO  - DOI:10.1021/acsnano.6b02627
UR  - https://juser.fz-juelich.de/record/826950
ER  -