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@INPROCEEDINGS{Streun:826975,
author = {Streun, M. and Pflugfelder, D. and van Dusschoten, D. and
Erven, A. and Jokhovets, L. and Kochs, J. and Metzner, R.
and Nöldgen, H. and Koller, R. and Scheins, J. and Postma,
Johannes Auke and Bühler, J. and Chlubek, A. and Jahnke, S.
and van Waasen, Stefan and Schurr, U.},
title = {{I}maging of {P}lants with {MRI} and the dedicated {PET}
scanner pheno{PET}},
reportid = {FZJ-2017-01182},
year = {2016},
abstract = {ABSTRACTWithin the German Plant Phenotyping Network (DPPN),
we develop Magnetic Resonance Imaging (MRI) and Positron
Emission Tomography (PET) as two modalities enabling a
noninvasive observation of the three-dimensional structural
and functional properties of roots grown in soil. The MRI
System is a 4.7T modified small animal scanner (Varian) and
produces 3D images with a high root to soil contrast [1]. A
user-friendly data processing pipeline has been established
to extract quantitative information out of these 3D MRI
images [2]. Functional information on carbon transport
within intact root systems can be obtained by positron
emission tomography. Radioactively labelled [11C]-CO2 is
taken up by photosynthesis and labelled carbon is
transported into the root system. In order to characterize
these transport mechanisms the new PET system phenoPET has
been developed in cooperation with Philips Digital Photon
Counting (PDPC). The scanner employs LYSO scintillators of
1.85×1.85×10 mm3 and digital SiPM arrays as photo
detectors (DPC3200-22-44, PDPC) [3] which are arranged in
three stacked rings [4]. The field of view measures 18 cm in
diameter and 20 cm of axial height. The phenoPET system has
been assembled and very first plant images have been
obtained using the PET image reconstruction software PRESTO
[5]. For a quantitative reconstruction of an arbitrary 3D
tracer distribution several correction steps still need to
be implemented. Thus, the phenoPET is expected to be
available for plant measurements by mid 2016.Combining both
modalities for a non-invasive structural (MRI) and
functional (PET) observation of roots grown in soil may
accelerate trait identification of resource efficient roots.
REFERENCES[1] Jahnke et al., “Combined MRI–PET dissects
dynamic changes in plant structures and functions”, The
Plant Journal 59, 634–644 (2009)[2] D. van Dusschoten et
al. „Quantitative 3D Analysis of Plant Roots growing in
Soil using Magnetic Resonance Imaging”, Plant Physiology
DOI:10.1104/pp.15.01388 (2016)[3] Y. Haemisch, T. Frach, C.
Degenhardt, and A. Thon, "Fully Digital Arrays of Silicon
Photomultipliers (dSiPM) – a Scalable Alternative to
Vacuum Photomultiplier Tubes (PMT)." Physics Procedia 37,
1546 (2012)[4] M. Streun et al., “phenoPET: A dedicated
PET Scanner for Plant Research based on digital SiPMs
(DPCs)”, NSS/MIC 2014, Seattle, Conf. Rec. M11-18
(2014)[5] Scheins, J., et al., Fully-3D PET Image
Reconstruction Using Scanner-Independent, Adaptive
Projection Dara and Highly Rotation-Symmetric Voxel
Assemblies, IEEE Transaction on Medical Imaging, Vol. 30,
No. 3, Mar 2011, pp. 879-892},
month = {May},
date = {2016-05-23},
organization = {PET/MR and SPECT/MR 2016, Köln
(Germany), 23 May 2016 - 25 May 2016},
subtyp = {After Call},
cin = {ZEA-2 / IBG-2-3-TA},
cid = {I:(DE-Juel1)ZEA-2-20090406 /
I:(DE-Juel1)IBG-2-3-TA-20110204},
pnm = {582 - Plant Science (POF3-582) / DPPN - Deutsches Pflanzen
Phänotypisierungsnetzwerk (BMBF-031A053A)},
pid = {G:(DE-HGF)POF3-582 / G:(DE-Juel1)BMBF-031A053A},
typ = {PUB:(DE-HGF)24},
url = {https://juser.fz-juelich.de/record/826975},
}