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000827054 1001_ $$0P:(DE-HGF)0$$aFischer, Niels$$b0$$eCorresponding author
000827054 245__ $$aThe pathway to GTPase activation of elongation factor SelB on the ribosome
000827054 260__ $$aLondon [u.a.]$$bNature Publ. Group$$c2016
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000827054 520__ $$aIn all domains of life, selenocysteine (Sec) is delivered to the ribosome by selenocysteine-specific tRNA (tRNASec) with the help of a specialized translation factor, SelB in bacteria. Sec-tRNASec recodes a UGA stop codon next to a downstream mRNA stem–loop. Here we present the structures of six intermediates on the pathway of UGA recoding in Escherichia coli by single-particle cryo-electron microscopy. The structures explain the specificity of Sec-tRNASec binding by SelB and show large-scale rearrangements of Sec-tRNASec. Upon initial binding of SelB–Sec-tRNASec to the ribosome and codon reading, the 30S subunit adopts an open conformation with Sec-tRNASec covering the sarcin–ricin loop (SRL) on the 50S subunit. Subsequent codon recognition results in a local closure of the decoding site, which moves Sec-tRNASec away from the SRL and triggers a global closure of the 30S subunit shoulder domain. As a consequence, SelB docks on the SRL, activating the GTPase of SelB. These results reveal how codon recognition triggers GTPase activation in translational GTPases.
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000827054 7001_ $$0P:(DE-HGF)0$$aNeumann, Piotr$$b1
000827054 7001_ $$0P:(DE-HGF)0$$aBock, Lars V.$$b2
000827054 7001_ $$0P:(DE-HGF)0$$aMaracci, Cristina$$b3
000827054 7001_ $$0P:(DE-Juel1)138909$$aWang, Zhe$$b4
000827054 7001_ $$0P:(DE-HGF)0$$aPaleskava, Alena$$b5
000827054 7001_ $$0P:(DE-HGF)0$$aKonevega, Andrey L.$$b6
000827054 7001_ $$0P:(DE-Juel1)132018$$aSchröder, Gunnar$$b7$$ufzj
000827054 7001_ $$0P:(DE-HGF)0$$aHelmut, Grubmüller$$b8
000827054 7001_ $$0P:(DE-HGF)0$$aFicner, Ralf$$b9
000827054 7001_ $$0P:(DE-HGF)0$$aRodnina, Marina V.$$b10
000827054 7001_ $$0P:(DE-HGF)0$$aStark, Holger$$b11$$eCorresponding author
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