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| Hauptseite > Publikationsdatenbank > The pathway to GTPase activation of elongation factor SelB on the ribosome > print |
| Hauptseite > Publikationsdatenbank > The pathway to GTPase activation of elongation factor SelB on the ribosome > print |
| 001 | 827054 | ||
| 005 | 20210129225740.0 | ||
| 024 | 7 | _ | |a 10.1038/nature20560 |2 doi |
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| 100 | 1 | _ | |0 P:(DE-HGF)0 |a Fischer, Niels |b 0 |e Corresponding author |
| 245 | _ | _ | |a The pathway to GTPase activation of elongation factor SelB on the ribosome |
| 260 | _ | _ | |a London [u.a.] |b Nature Publ. Group |c 2016 |
| 336 | 7 | _ | |2 DRIVER |a article |
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| 520 | _ | _ | |a In all domains of life, selenocysteine (Sec) is delivered to the ribosome by selenocysteine-specific tRNA (tRNASec) with the help of a specialized translation factor, SelB in bacteria. Sec-tRNASec recodes a UGA stop codon next to a downstream mRNA stem–loop. Here we present the structures of six intermediates on the pathway of UGA recoding in Escherichia coli by single-particle cryo-electron microscopy. The structures explain the specificity of Sec-tRNASec binding by SelB and show large-scale rearrangements of Sec-tRNASec. Upon initial binding of SelB–Sec-tRNASec to the ribosome and codon reading, the 30S subunit adopts an open conformation with Sec-tRNASec covering the sarcin–ricin loop (SRL) on the 50S subunit. Subsequent codon recognition results in a local closure of the decoding site, which moves Sec-tRNASec away from the SRL and triggers a global closure of the 30S subunit shoulder domain. As a consequence, SelB docks on the SRL, activating the GTPase of SelB. These results reveal how codon recognition triggers GTPase activation in translational GTPases. |
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| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Neumann, Piotr |b 1 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Bock, Lars V. |b 2 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Maracci, Cristina |b 3 |
| 700 | 1 | _ | |0 P:(DE-Juel1)138909 |a Wang, Zhe |b 4 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Paleskava, Alena |b 5 |
| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Konevega, Andrey L. |b 6 |
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| 700 | 1 | _ | |0 P:(DE-HGF)0 |a Stark, Holger |b 11 |e Corresponding author |
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