TY  - JOUR
AU  - Kube, Sarah
AU  - Hersch, Nils
AU  - Naumovska, Elena
AU  - Gensch, Thomas
AU  - Hendriks, Johnny
AU  - Franzen, Arne
AU  - Landvogt, Lisa
AU  - Siebrasse, Jan-Peter
AU  - Kubitscheck, Ulrich
AU  - Hoffmann, Bernd
AU  - Merkel, Rudolf
AU  - Csiszár, Agnes
TI  - Fusogenic Liposomes as Nanocarriers for the Delivery of Intracellular Proteins
JO  - Langmuir
VL  - 33
IS  - 4
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2017-01888
SP  - 1051 - 1059
PY  - 2017
AB  - Direct delivery of proteins and peptides into living mammalian cells has been accomplished using phospholipid liposomes as carrier particles. Such liposomes are usually taken up via endocytosis where the main part of their cargo is degraded in lysosomes before reaching its destination. Here, fusogenic liposomes, a newly developed molecular carrier system, were used for protein delivery. When such liposomes were loaded with water-soluble proteins and brought into contact with mammalian cells, the liposomal membrane efficiently fused with the cellular plasma membrane delivering the liposomal content to the cytoplasm without degradation. To explore the key factors of proteofection processes, the complex formation of fusogenic liposomes and proteins of interest and the size and zeta potential of the formed fusogenic proteoliposoms were monitored. Intracellular protein delivery was analyzed using fluorescence microscopy and flow cytometry. Proteins such as EGFP, Dendra2, and R-phycoerythrin or peptides such as LifeAct-FITC and NTF2-AlexaFluor488 were successfully incorporated into mammalian cells with high efficiency. Moreover, correct functionality and faithful transport to binding sites were also proven for the imported proteins.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000393269700026
C6  - pmid:28059515
DO  - DOI:10.1021/acs.langmuir.6b04304
UR  - https://juser.fz-juelich.de/record/827784
ER  -