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@ARTICLE{Fasbender:827951,
author = {Fasbender, Stefan and Allani, Sonja and Wimmenauer,
Christian and Cadeddu, Ron-Patrick and Raba, Katharina and
Fischer, Johannes C. and Bulat, Bekir and Luysberg, Martina
and Seidel, Claus A. M. and Heinzel, Thomas and Haas,
Rainer},
title = {{U}ptake dynamics of graphene quantum dots into primary
human blood cells following in vitro exposure},
journal = {RSC Advances},
volume = {7},
number = {20},
issn = {2046-2069},
address = {London},
publisher = {RSC Publishing},
reportid = {FZJ-2017-01983},
pages = {12208 - 12216},
year = {2017},
abstract = {Human leukocytes obtained from samples of leukapheresis
products of three healthy donors stimulated by granulocyte
colony stimulating factor (G-CSF) were exposed to graphene
quantum dots. A time- and concentration dependent uptake was
observed with a significantly greater uptake into monocytes
and granulocytes in comparison to lymphocytes, suggesting a
better incorporation ability of cells with phagocytotic
properties. The uptake rates also correlate with the cell
membrane area. Looking at the different lymphoid subsets a
greater uptake was found into CD19+ B-, CD56+ natural killer
cells and CD34+ hematopoietic stem cells (HSC) in comparison
to CD4+ T- and CD8+ T cells. Independent of the cell type
studied, the observed uptake dynamics is consistent with a
diffusion-driven process, which allows the determination of
cell-specific membrane permeabilities for the graphene
quantum dots. The toxicity of the quantum dots is relatively
low resulting in a $90\%$ viability of the entire leukocyte
population after 36 hours of exposure to GQDs at a
concentration of 500 μg ml−1.},
cin = {ER-C-1},
ddc = {540},
cid = {I:(DE-Juel1)ER-C-1-20170209},
pnm = {143 - Controlling Configuration-Based Phenomena (POF3-143)},
pid = {G:(DE-HGF)POF3-143},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000395873500047},
doi = {10.1039/C6RA27829A},
url = {https://juser.fz-juelich.de/record/827951},
}