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@ARTICLE{Moudkov:827998,
      author       = {Moudříková, Šárka and Nedbal, Ladislav and
                      Solovchenko, Alexei and Mojzeš, Peter},
      title        = {{R}aman microscopy shows that nitrogen-rich cellular
                      inclusions in microalgae are microcrystalline guanine},
      journal      = {Algal Research},
      volume       = {23},
      issn         = {2211-9264},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier},
      reportid     = {FZJ-2017-02020},
      pages        = {216 - 222},
      year         = {2017},
      abstract     = {Microalgal cells possess a vast diversity of subcellular
                      structures and cytoplasmic inclusions differing in
                      theirmorphology, functionality, and composition, some of
                      them giving rise to distinct Raman spectral
                      signaturesallowing their identification, localization, and
                      visualization in situ. Here,we showthat certain Raman
                      features observedin Raman spectra of microalgae can be
                      unambiguously attributed to guanine microcrystals because
                      theyare clearly distinct from Raman fingerprints of closely
                      related purine species. Using confocal Raman microscopy,we
                      have localized crystalline guanine as a part of cellular
                      inclusions in the chlorophyte Desmodesmus quadricaudaand in
                      the eustigmatophyte Trachydiscus minutus. Wepropose that
                      this finding also explains the chemical natureof similar
                      nitrogen-rich crystalline structures recently documented in
                      a number of other chlorophyte species byenergy-dispersive
                      X-ray spectroscopy. To the best of our knowledge, this is
                      the first Raman microscopy-based directevidence of the
                      presence of guanine microcrystalline inclusions within
                      microalgal cells. We tentatively proposethat the crystalline
                      guanine serves as a very compact, long-termdepot of nitrogen
                      in microalgae. Simplicityof specimen preparation requiring
                      no fixation, labeling, or staining of the cells
                      predetermines Raman microscopyas a method of choice for more
                      advanced studies of the physiological role of guanine
                      particles, as well as othercrystalline inclusions in situ
                      within intact cells.},
      cin          = {IBG-2},
      ddc          = {580},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582)},
      pid          = {G:(DE-HGF)POF3-582},
      typ          = {PUB:(DE-HGF)16},
      UT           = {WOS:000397462700024},
      doi          = {10.1016/j.algal.2017.02.009},
      url          = {https://juser.fz-juelich.de/record/827998},
}