TY  - JOUR
AU  - Wojciechowski, Kamil
AU  - Orczyk, Marta
AU  - Gutberlet, Thomas
AU  - Brezesinski, Gerald
AU  - Geue, Thomas
AU  - Fontaine, Philippe
TI  - On the Interaction between Digitonin and Cholesterol in Langmuir Monolayers
JO  - Langmuir
VL  - 32
IS  - 35
SN  - 1520-5827
CY  - Washington, DC
PB  - ACS Publ.
M1  - FZJ-2017-02021
SP  - 9064 - 9073
PY  - 2016
AB  - In this article, we describe the effect of a highly hemolytic saponin, digitonin, on model lipids cholesterol and dipalmitoylphosphatidylcholine (DPPC) using a combination of tensiometric (surface pressure and dilatational surface elasticity), spectroscopic (infrared reflection absorption spectroscopy, IRRAS), microscopic (fluorescence microscopy), and scattering techniques (neutron reflectivity, NR, and grazing incidence X-ray diffraction, GIXD). The monolayers of individual lipids and their 10:9 (mol/mol) mixture were exposed to an aqueous solution of digitonin (10–4 M) by subphase exchange using a setup developed recently in our laboratory. The results confirm that digitonin can adsorb onto both bare and lipid-covered water–air interfaces. In the case of DPPC, a relatively weak interaction can be observed, but the presence of cholesterol drastically enhances the effect of digitonin. The latter is shown to dissociate the weak cholesterol–DPPC complexes and to bind cholesterol in an additional layer attached to the original lipid monolayer.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000382805700033
C6  - pmid:27518122
DO  - DOI:10.1021/acs.langmuir.6b01737
UR  - https://juser.fz-juelich.de/record/827999
ER  -