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@ARTICLE{Wojciechowski:827999,
author = {Wojciechowski, Kamil and Orczyk, Marta and Gutberlet,
Thomas and Brezesinski, Gerald and Geue, Thomas and
Fontaine, Philippe},
title = {{O}n the {I}nteraction between {D}igitonin and
{C}holesterol in {L}angmuir {M}onolayers},
journal = {Langmuir},
volume = {32},
number = {35},
issn = {1520-5827},
address = {Washington, DC},
publisher = {ACS Publ.},
reportid = {FZJ-2017-02021},
pages = {9064 - 9073},
year = {2016},
abstract = {In this article, we describe the effect of a highly
hemolytic saponin, digitonin, on model lipids cholesterol
and dipalmitoylphosphatidylcholine (DPPC) using a
combination of tensiometric (surface pressure and
dilatational surface elasticity), spectroscopic (infrared
reflection absorption spectroscopy, IRRAS), microscopic
(fluorescence microscopy), and scattering techniques
(neutron reflectivity, NR, and grazing incidence X-ray
diffraction, GIXD). The monolayers of individual lipids and
their 10:9 (mol/mol) mixture were exposed to an aqueous
solution of digitonin (10–4 M) by subphase exchange using
a setup developed recently in our laboratory. The results
confirm that digitonin can adsorb onto both bare and
lipid-covered water–air interfaces. In the case of DPPC, a
relatively weak interaction can be observed, but the
presence of cholesterol drastically enhances the effect of
digitonin. The latter is shown to dissociate the weak
cholesterol–DPPC complexes and to bind cholesterol in an
additional layer attached to the original lipid monolayer.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1 /
JCNS-2},
ddc = {670},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106 / I:(DE-Juel1)JCNS-2-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)NOSPEC-20140101},
typ = {PUB:(DE-HGF)16},
UT = {WOS:000382805700033},
pubmed = {pmid:27518122},
doi = {10.1021/acs.langmuir.6b01737},
url = {https://juser.fz-juelich.de/record/827999},
}