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000828004 1001_ $$00000-0002-1700-4759$$aDa Vela, Stefano$$b0
000828004 245__ $$aKinetics of liquid–liquid phase separation in protein solutions exhibiting LCST phase behavior studied by time-resolved USAXS and VSANS
000828004 260__ $$aLondon$$bRoyal Soc. of Chemistry$$c2016
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000828004 520__ $$aWe study the kinetics of the liquid–liquid phase separation (LLPS) and its arrest in protein solutions exhibiting a lower critical solution temperature (LCST) phase behavior using the combination of ultra-small angle X-ray scattering (USAXS) and very-small angle neutron scattering (VSANS). We employ a previously established model system consisting of bovine serum albumin (BSA) solutions with YCl3. We follow the phase transition from sub-second to 104 s upon an off-critical temperature jump. After a temperature jump, the USAXS profiles exhibit a peak that grows in intensity and shifts to lower q values with time. Below 45 °C, the characteristic length scale (ξ) obtained from this scattering peak increases with time with a power of about 1/3 for different sample compositions. This is in good agreement with the theoretical prediction for the intermediate stage of spinodal decomposition where the growth is driven by interface tension. Above 45 °C, ξ follows initially the 1/3 power law growth, then undergoes a significant slowdown, and an arrested state is reached below the denaturation temperature of the protein. This growth kinetics may indicate that the final composition of the protein-rich phase is located close to the high density branch of the LLPS binodal when a kinetically arrested state is reached.
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000828004 65017 $$0V:(DE-MLZ)GC-1602-2016$$2V:(DE-HGF)$$aPolymers, Soft Nano Particles and  Proteins$$x0
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000828004 7001_ $$0P:(DE-HGF)0$$aBraun, Michal K.$$b1
000828004 7001_ $$0P:(DE-HGF)0$$aDörr, Andreas$$b2
000828004 7001_ $$0P:(DE-HGF)0$$aGreco, Alessandro$$b3
000828004 7001_ $$00000-0001-8363-9077$$aMöller, Johannes$$b4
000828004 7001_ $$0P:(DE-Juel1)130647$$aFu, Zhendong$$b5$$ufzj
000828004 7001_ $$0P:(DE-HGF)0$$aZhang, Fajun$$b6$$eCorresponding author
000828004 7001_ $$0P:(DE-HGF)0$$aSchreiber, Frank$$b7
000828004 773__ $$0PERI:(DE-600)2191476-X$$a10.1039/C6SM01837H$$gVol. 12, no. 46, p. 9334 - 9341$$n46$$p9334 - 9341$$tSoft matter$$v12$$x1744-6848$$y2016
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