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| 001 | 828004 | ||
| 005 | 20210129225951.0 | ||
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| 100 | 1 | _ | |a Da Vela, Stefano |0 0000-0002-1700-4759 |b 0 |
| 245 | _ | _ | |a Kinetics of liquid–liquid phase separation in protein solutions exhibiting LCST phase behavior studied by time-resolved USAXS and VSANS |
| 260 | _ | _ | |a London |c 2016 |b Royal Soc. of Chemistry |
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| 520 | _ | _ | |a We study the kinetics of the liquid–liquid phase separation (LLPS) and its arrest in protein solutions exhibiting a lower critical solution temperature (LCST) phase behavior using the combination of ultra-small angle X-ray scattering (USAXS) and very-small angle neutron scattering (VSANS). We employ a previously established model system consisting of bovine serum albumin (BSA) solutions with YCl3. We follow the phase transition from sub-second to 104 s upon an off-critical temperature jump. After a temperature jump, the USAXS profiles exhibit a peak that grows in intensity and shifts to lower q values with time. Below 45 °C, the characteristic length scale (ξ) obtained from this scattering peak increases with time with a power of about 1/3 for different sample compositions. This is in good agreement with the theoretical prediction for the intermediate stage of spinodal decomposition where the growth is driven by interface tension. Above 45 °C, ξ follows initially the 1/3 power law growth, then undergoes a significant slowdown, and an arrested state is reached below the denaturation temperature of the protein. This growth kinetics may indicate that the final composition of the protein-rich phase is located close to the high density branch of the LLPS binodal when a kinetically arrested state is reached. |
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| 700 | 1 | _ | |a Dörr, Andreas |0 P:(DE-HGF)0 |b 2 |
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| 700 | 1 | _ | |a Möller, Johannes |0 0000-0001-8363-9077 |b 4 |
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| 700 | 1 | _ | |a Zhang, Fajun |0 P:(DE-HGF)0 |b 6 |e Corresponding author |
| 700 | 1 | _ | |a Schreiber, Frank |0 P:(DE-HGF)0 |b 7 |
| 773 | _ | _ | |a 10.1039/C6SM01837H |g Vol. 12, no. 46, p. 9334 - 9341 |0 PERI:(DE-600)2191476-X |n 46 |p 9334 - 9341 |t Soft matter |v 12 |y 2016 |x 1744-6848 |
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