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000828252 037__ $$aFZJ-2017-02220
000828252 041__ $$aEnglish
000828252 1001_ $$0P:(DE-Juel1)129471$$aHofmann, Diana$$b0$$eCorresponding author$$ufzj
000828252 1112_ $$a1st IWA Conference on Algal Technologies for Wastewater Treatment and Resource Recovery$$cDelft$$d2017-03-16 - 2017-03-17$$wThe Netherlands
000828252 245__ $$a33P-Labeling of chlorella vulgaris algae for methodical studies
000828252 260__ $$c2017
000828252 3367_ $$033$$2EndNote$$aConference Paper
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000828252 3367_ $$0PUB:(DE-HGF)24$$2PUB:(DE-HGF)$$aPoster$$bposter$$mposter$$s1490684274_14631$$xPlenary/Keynote
000828252 520__ $$aPhosphorus (P), a major nutrient for plants, is a finite non-renewable resource of modern agriculture. Nevertheless, the efficiency of P usage today hardly reaches 20% with the rest ending in wastewater or carried away by runoff from fields to rivers and to the oceans. In our study, we will investigate the potential to close the cycle from waste back to agriculture by exploiting the capability of microalgae to accumulate large P quantities. This potential of algae for a ‘luxury P uptake’ will be combined with the benefit of delayed release of P from the algal biomass applied as a fertilizer to soil. To achieve our goals, first we selected best suitable algae subspecies of Clorella vulgaris with regard to maximal P incorporation. Afterwards, the pre-culture conditions were optimized in regard of tracer uptake. In several experiments we compared the behavior of washed and different starved out algae in the following, simple experimental setup: erlenmayer flasks with algae in radioactive labelled nutrition solution (in form of orthophosphate) were gently shaked in an incubator under appropriated illumination. In various time intervals sampling occurs and the aliquots were centrifuged. The sedimented, labelled algae were digested with concentrated HNO3. Thereafter, both, the digested solutions derived from the sediments as well as the supernatants containing still free orthophosphate were measured in the liquid scintillation counter (LSC) for their activities. Effects of lighting were additional investigated as well as the possibility to label greater amounts by means of using algae different optical depths. As result, main influence to achieve fast and possibly complete incorporation of the label is an extreme low, but not missing phosphorous content in the nutrition solution, as we studied by systematic investigations using hungry algae out of the pre-culture. In future, (in such way) labelled algae shall be used for rhizotrone experiments to study how P in algae derived fertilizer deploys to the soil and is taken up by wheat plants by means of bioimaging. Furthermore, soil aliquots as well as different plant parts shall be digested after harvest and measured in LCS for quantitation purposes.
000828252 536__ $$0G:(DE-HGF)POF3-255$$a255 - Terrestrial Systems: From Observation to Prediction (POF3-255)$$cPOF3-255$$fPOF III$$x0
000828252 7001_ $$0P:(DE-Juel1)165137$$aSchiedung, Henning$$b1$$ufzj
000828252 7001_ $$0P:(DE-Juel1)159104$$aSchreiber, Christina$$b2$$ufzj
000828252 7001_ $$0P:(DE-Juel1)168528$$aAckermann, Bärbel$$b3$$ufzj
000828252 7001_ $$0P:(DE-Juel1)159592$$aNedbal, Ladislav$$b4$$ufzj
000828252 7001_ $$0P:(DE-Juel1)129427$$aAmelung, Wulf$$b5$$ufzj
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000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129471$$aForschungszentrum Jülich$$b0$$kFZJ
000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)165137$$aForschungszentrum Jülich$$b1$$kFZJ
000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)159104$$aForschungszentrum Jülich$$b2$$kFZJ
000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)168528$$aForschungszentrum Jülich$$b3$$kFZJ
000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)159592$$aForschungszentrum Jülich$$b4$$kFZJ
000828252 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)129427$$aForschungszentrum Jülich$$b5$$kFZJ
000828252 9131_ $$0G:(DE-HGF)POF3-255$$1G:(DE-HGF)POF3-250$$2G:(DE-HGF)POF3-200$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bErde und Umwelt$$lTerrestrische Umwelt$$vTerrestrial Systems: From Observation to Prediction$$x0
000828252 9141_ $$y2017
000828252 920__ $$lyes
000828252 9201_ $$0I:(DE-Juel1)IBG-3-20101118$$kIBG-3$$lAgrosphäre$$x0
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