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@INPROCEEDINGS{Hofmann:828252,
      author       = {Hofmann, Diana and Schiedung, Henning and Schreiber,
                      Christina and Ackermann, Bärbel and Nedbal, Ladislav and
                      Amelung, Wulf},
      title        = {33{P}-{L}abeling of chlorella vulgaris algae for methodical
                      studies},
      reportid     = {FZJ-2017-02220},
      year         = {2017},
      abstract     = {Phosphorus (P), a major nutrient for plants, is a finite
                      non-renewable resource of modern agriculture. Nevertheless,
                      the efficiency of P usage today hardly reaches $20\%$ with
                      the rest ending in wastewater or carried away by runoff from
                      fields to rivers and to the oceans. In our study, we will
                      investigate the potential to close the cycle from waste back
                      to agriculture by exploiting the capability of microalgae to
                      accumulate large P quantities. This potential of algae for a
                      ‘luxury P uptake’ will be combined with the benefit of
                      delayed release of P from the algal biomass applied as a
                      fertilizer to soil. To achieve our goals, first we selected
                      best suitable algae subspecies of Clorella vulgaris with
                      regard to maximal P incorporation. Afterwards, the
                      pre-culture conditions were optimized in regard of tracer
                      uptake. In several experiments we compared the behavior of
                      washed and different starved out algae in the following,
                      simple experimental setup: erlenmayer flasks with algae in
                      radioactive labelled nutrition solution (in form of
                      orthophosphate) were gently shaked in an incubator under
                      appropriated illumination. In various time intervals
                      sampling occurs and the aliquots were centrifuged. The
                      sedimented, labelled algae were digested with concentrated
                      HNO3. Thereafter, both, the digested solutions derived from
                      the sediments as well as the supernatants containing still
                      free orthophosphate were measured in the liquid
                      scintillation counter (LSC) for their activities. Effects of
                      lighting were additional investigated as well as the
                      possibility to label greater amounts by means of using algae
                      different optical depths. As result, main influence to
                      achieve fast and possibly complete incorporation of the
                      label is an extreme low, but not missing phosphorous content
                      in the nutrition solution, as we studied by systematic
                      investigations using hungry algae out of the pre-culture. In
                      future, (in such way) labelled algae shall be used for
                      rhizotrone experiments to study how P in algae derived
                      fertilizer deploys to the soil and is taken up by wheat
                      plants by means of bioimaging. Furthermore, soil aliquots as
                      well as different plant parts shall be digested after
                      harvest and measured in LCS for quantitation purposes.},
      month         = {Mar},
      date          = {2017-03-16},
      organization  = {1st IWA Conference on Algal
                       Technologies for Wastewater Treatment
                       and Resource Recovery, Delft (The
                       Netherlands), 16 Mar 2017 - 17 Mar
                       2017},
      subtyp        = {Plenary/Keynote},
      cin          = {IBG-3},
      cid          = {I:(DE-Juel1)IBG-3-20101118},
      pnm          = {255 - Terrestrial Systems: From Observation to Prediction
                      (POF3-255)},
      pid          = {G:(DE-HGF)POF3-255},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/828252},
}