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@INPROCEEDINGS{Hofmann:828386,
      author       = {Hofmann, Diana and Schiedung, Henning and Ackermann,
                      Bärbel and Schreiber, Christina and Nedbal, Ladislav and
                      Amelung, Wulf},
      title        = {33{P} {L}abeling of {C}hlorella vulgaris algae for
                      methodical studies},
      reportid     = {FZJ-2017-02347},
      year         = {2017},
      abstract     = {Phosphorus (P), a major nutrient for plants, is a finite
                      non-renewable resource of modern agriculture. Nevertheless,
                      the efficiency of P usage today hardly reaches $20\%$ with
                      the rest ending in wastewater or carried away by runoff from
                      fields to rivers and to the oceans. In our study, we will
                      investigate the potential to close the cycle from waste back
                      to agriculture by exploiting the capability of microalgae to
                      accumulate large P quantities. This potential of algae for a
                      ‘luxury P uptake’ will be combined with the benefit of
                      delayed release of P from the algal biomass applied as a
                      fertilizer to soil. To achieve our goals, first we selected
                      best suitable algae with regard to maximal P incorporation.
                      Afterwards, the pre-culture conditions were optimized. In
                      several experiments we compared the behavior of washed and
                      different starved out algae in the following, simple
                      experimental setup: erlenmayer flasks with algae in
                      radioactive labelled nutrition solution (in form of
                      orthophosphate) were gently shaked in an incubator under
                      appropriated illumination. In various time intervals
                      sampling occurs and the aliquots were centrifuged. The
                      sedimented, labelled algae were digested with concentrated
                      HNO3. Thereafter, both, the digested solutions as well as
                      the supernatants containing still free orthophosphate were
                      measured in the liquid scintillation counter (LSC) for their
                      activities. Effects of lighting were additional investigated
                      as well as the possibility to label greater amounts by means
                      of using algae different optical depths. As result, main
                      influence to achieve fast and possibly complete
                      incorporation of the label is an extreme low, but not
                      missing phosphorous content in the nutrition solution, as we
                      studied by systematic investigations using hungry algae out
                      of the pre-culture. In future, in such way labelled algae
                      shall be used for rhizotrone experiments to study how P in
                      algae derived fertilizer deploys to the soil and is taken up
                      by wheat plants by means of bioimaging. Furthermore, soil
                      aliquots as well as different plant parts were digested
                      after harvest and measured in LCS for quantitation
                      purposes.},
      month         = {Mar},
      date          = {2017-03-16},
      organization  = {IWA Conference on Algal Technologies
                       for Wastewater Treatment and Resource
                       Recovery, Delft (The Netherlands), 16
                       Mar 2017 - 17 Mar 2017},
      subtyp        = {Other},
      cin          = {IBG-2},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582) / AF AlgalFertilizer -
                      AlgalFertilizer (20172303) / BioSC - Bioeconomy Science
                      Center (BioSC)},
      pid          = {G:(DE-HGF)POF3-582 / G:(BioSC)20172303 / G:(DE-Juel1)BioSC},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/828386},
}